Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital Indonesia, Jakarta, Indonesia.
Biomed Res Int. 2021 Feb 23;2021:8824614. doi: 10.1155/2021/8824614. eCollection 2021.
An increase of steroid hormones in controlled ovarian hyperstimulation (COH) procedures is reducing the success rate in assisted reproductive technology (ART), and this includes the pregnancy rate and/or implantation rate. Research has found that the decrease in the success rate occurred due to the decreased expression of the protein that is needed to prepare the endometrium so that the embryo could attach. The aim of the study was to analyse the changes in E-chaderin expression due to COH and its relations with increased level of steroid hormones as one of the proteins in the endometrium. There were 13 samples of stored biological tissue from endometrial tissue; came from one group of natural cycles as the control group ( = 4) and three groups of stimulated cycles. The first stimulated cycle group was injected by a 30 IU dose of rFSH ( = 2). The second stimulated cycle group was injected by a 50 IU dose of rFSH ( = 4). The third stimulated cycle group was injected by a 70 IU dose of rFSH ( = 3). The expression of E-cadherin was measured by the immunohistochemistry (IHC) technique. Estradiol (E2) and progesterone (P4) levels were assessed using ELISA and have already been done. The IHC staining expression of E-cadherin was found in the cytoplasm of glandular epithelium. Immunostaining measurement used the H_SCORE. We found that the expression of E-cadherin within the group was not significantly different ( value: 0.178). Similarly, both the correlation between the estradiol level with E-cadherin and the correlation between the progesterone level with E-cadherin were not significantly different ( value: 0.872 and value: 0.836). The conclusion is that the level of E-Cadherin expression in the endometrium that were taken in themiddle secretion phase not affected by the dose regimen that given. In addition, the level of expression is not influenced by the increase of serum E2 and P4 levels.
控制性卵巢超刺激（COH）过程中类固醇激素的增加降低了辅助生殖技术（ART）的成功率，包括妊娠率和/或着床率。研究发现，成功率下降是由于准备子宫内膜所需的蛋白质表达减少，从而使胚胎无法附着。本研究旨在分析 COH 导致的 E-钙粘蛋白表达的变化及其与类固醇激素水平升高的关系，E-钙粘蛋白是子宫内膜中的一种蛋白质。有 13 个来自子宫内膜组织的储存生物组织样本；来自自然周期的一组作为对照组（=4）和三组刺激周期。第一组刺激周期注射 30IU 的 rFSH（=2）。第二组刺激周期注射 50IU 的 rFSH（=4）。第三组刺激周期注射 70IU 的 rFSH（=3）。E-钙粘蛋白的表达通过免疫组织化学（IHC）技术测量。雌二醇（E2）和孕酮（P4）水平使用 ELISA 进行评估，已经完成。E-钙粘蛋白的 IHC 染色表达在腺上皮的细胞质中发现。免疫染色测量使用 H_SCORE。我们发现组内 E-钙粘蛋白的表达没有显著差异（ 值：0.178）。同样，雌二醇水平与 E-钙粘蛋白之间的相关性和孕酮水平与 E-钙粘蛋白之间的相关性均无显著差异（ 值：0.872 和 值：0.836）。结论是，在分泌中期采集的子宫内膜中 E-钙粘蛋白的表达水平不受所给剂量方案的影响。此外，表达水平不受血清 E2 和 P4 水平升高的影响。
