Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Adv Mater. 2021 Apr;33(17):e2007473. doi: 10.1002/adma.202007473. Epub 2021 Mar 11.
Engineered probiotics have the potential to diagnose and treat a variety of gastrointestinal (GI) diseases. However, these exogenous bacterial agents have limited ability to effectively colonize specific regions of the GI tract due to a lack of external control over their localization and persistence. Magnetic fields are well suited to providing such control, since they freely penetrate biological tissues. However, they are difficult to apply with sufficient strength to directly manipulate magnetically labeled cells in deep tissue such as the GI tract. Here, it is demonstrated that a composite biomagnetic material consisting of microscale magnetic particles and probiotic bacteria, when orally administered and combined with an externally applied magnetic field, enables the trapping and retention of probiotic bacteria within the GI tract of mice. This technology improves the ability of these probiotic agents to accumulate at specific locations and stably colonize without antibiotic treatment. By enhancing the ability of GI-targeted probiotics to be at the right place at the right time, cellular localization assisted by magnetic particles (CLAMP) adds external physical control to an important emerging class of microbial theranostics.
工程益生菌具有诊断和治疗多种胃肠道（GI）疾病的潜力。然而，由于缺乏对其定位和持久性的外部控制，这些外源性细菌制剂在有效定殖胃肠道特定区域方面的能力有限。磁场非常适合提供这种控制，因为它们可以自由穿透生物组织。然而，由于难以施加足够的强度直接在胃肠道等深部组织中操纵磁性标记的细胞，因此很难应用磁场。在这里，证明了由微尺度磁性颗粒和益生菌细菌组成的复合生物磁材料，当口服给予并与外部施加的磁场结合使用时，能够将益生菌细菌捕获并保留在小鼠的胃肠道内。这项技术提高了这些益生菌制剂在没有抗生素治疗的情况下，在特定位置积累和稳定定植的能力。通过增强胃肠道靶向益生菌在适当的时间出现在适当位置的能力，磁性颗粒辅助的细胞定位（CLAMP）为一类新兴的微生物治疗学添加了外部物理控制。
