School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Anal Methods. 2021 Mar 28;13(12):1517-1526. doi: 10.1039/d0ay02253e. Epub 2021 Mar 12.
This paper describes a near-infrared quantum dot (CuInS QD)/antibiotic (vancomycin) nanoparticle-based assay for the Staphylococcus aureus and iron(iii) detection. CuInS QDs with good biological tissue permeability and biocompatibility are combined with vancomycin through covalent interaction to form a detection system for two harmful factors. The detection principle of Staphylococcus aureus is mainly the fluorescence quenching caused by the accumulation of CuInS@Van QDs on the surface of Staphylococcus aureus. The detection principles of the iron(iii) ion are mainly ascribed to the aggregation of quantum dots and the transfer of charges, which cause the fluorescence signal to change. The linear range of S. aureus and the Fe ion is 10 to 10 CFU mL and 10-90 μM, respectively. Their detection limits are 665 CFU mL and 3.5 μM, respectively. The procedure was validated by the quantitation of Staphylococcus aureus and iron(iii) in spiked samples, and was found to demonstrate the feasibility of this method.
本文描述了一种基于近红外量子点（CuInS QD）/抗生素（万古霉素）纳米颗粒的金黄色葡萄球菌和铁（III）检测方法。具有良好生物组织通透性和生物相容性的 CuInS QD 通过共价相互作用与万古霉素结合，形成针对两种有害因素的检测系统。金黄色葡萄球菌的检测原理主要是由于 CuInS@Van QDs 在金黄色葡萄球菌表面的积累引起的荧光猝灭。铁（III）离子的检测原理主要归因于量子点的聚集和电荷转移，导致荧光信号发生变化。金黄色葡萄球菌和 Fe 离子的线性范围分别为 10 到 10 CFU mL 和 10-90 μM，检测限分别为 665 CFU mL 和 3.5 μM。通过对加标样品中金黄色葡萄球菌和铁（III）的定量验证了该程序，证明了该方法的可行性。
