Furuno Masaaki, Atsumi Tatsuo, Yamada Taku, Kojima Seiji, Nishioka Noriko, Kawagishi Ikuro, Homma Michio
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan.
Department of Physics, Suzuka College of Technology, Shiroko-cho, Suzuka, Mie 510-02, Japan.
Microbiology (Reading). 1997 May;143(5):1615-1621. doi: 10.1099/00221287-143-5-1615.
has two types of flagella, polar (Pof) and lateral (Laf). From a Laf-defective mutant (PofLaf), polar-flagellar-length mutants which have short Pof and long Pof were isolated. The mean lengths of the helical axis in wild-type, short and long Pof were 5.5.0.9 μm, 2.5.0.6 μm and 11.2.3.6 μm, respectively. The swimming speeds of the short- and long-Pof mutants were slower than that of the wild-type strain. The relationship between swimming speed and flagellar length in a population of mutant cells was examined. In the short-Pof mutant, the decrease of swimming speed seemed to be derived from the decrease in flagellar length. In the long-Pof mutant, there was almost no correlation between swimming speed and flagellar length, and the slow swimming was explained by the helical shape of the flagella, whose pitch and radius were 1.4 μm and 0.062 μm, respectively, whereas those of the wild-type flagella were 1.5 μm and 0.16 μm. The relative amounts of the various molecular components of the long Pof were different from those of the wild-type or the short Pof. This seems to be the reason for the difference in flagellar shape and length, though the mutation may be pleiotropic and affect flagellar function or regulation.
有两种类型的鞭毛，极鞭（Pof）和侧鞭（Laf）。从一个Laf缺陷型突变体（PofLaf）中，分离出了极鞭长度突变体，其中包括短极鞭和长极鞭突变体。野生型、短极鞭和长极鞭的螺旋轴平均长度分别为5.5±0.9μm、2.5±0.6μm和11.2±3.6μm。短极鞭和长极鞭突变体的游动速度比野生型菌株慢。研究了突变体细胞群体中游泳速度与鞭毛长度之间的关系。在短极鞭突变体中，游泳速度的降低似乎源于鞭毛长度的减少。在长极鞭突变体中，游泳速度与鞭毛长度几乎没有相关性，慢速游动是由鞭毛的螺旋形状解释的，其螺距和半径分别为1.4μm和0.062μm，而野生型鞭毛的螺距和半径分别为1.5μm和0.16μm。长极鞭的各种分子成分的相对含量与野生型或短极鞭不同。这似乎是鞭毛形状和长度差异的原因，尽管该突变可能是多效性的，并影响鞭毛功能或调节。
