Barry B E, Mercer R R, Miller F J, Crapo J D
Department of Medicine, Duke University, Durham, North Carolina 27707.
Exp Lung Res. 1988;14(2):225-45. doi: 10.3109/01902148809115126.
Cells of the terminal bronchioles are particularly susceptible to the effects of inhalation of low levels of ozone (O3). One-day-old (juvenile) or 6-week-old (adult) rats were exposed to 0.25 ppm O3 for 12 h/day or to continuous room air for 6 weeks. Morphometric analysis of perpendicular cross sections of terminal bronchioles demonstrated that exposure to O3 produced alterations in the surface characteristics of ciliated and nonciliated (Clara) cells in both groups of rats. There were significant losses (20-30%) of the surface area contributed by cilia and the luminal surface of Clara cells was decreased by 16-25%. O3 exposure also produced significant decreases in the number of brush cells per square millimeter of terminal bronchiolar basement membrane. The results of this study indicate that the normal structure of terminal bronchiolar epithelial cells is significantly altered by inhalation of 0.25 ppm O3. No statistically significant interactions between the effects of O3 and animal age at the beginning of the exposure were found.
终末细支气管的细胞对吸入低水平臭氧（O₃）的影响尤为敏感。将1日龄（幼年）或6周龄（成年）大鼠每天暴露于0.25 ppm O₃中12小时，或暴露于持续的室内空气中6周。对终末细支气管垂直横截面的形态计量学分析表明，暴露于O₃会使两组大鼠的纤毛细胞和非纤毛（克拉拉）细胞的表面特征发生改变。纤毛贡献的表面积显著减少（20%-30%），克拉拉细胞的管腔表面积减少了16%-25%。暴露于O₃还使每平方毫米终末细支气管基底膜的刷细胞数量显著减少。本研究结果表明，吸入0.25 ppm O₃会使终末细支气管上皮细胞的正常结构发生显著改变。在暴露开始时，未发现O₃的影响与动物年龄之间存在统计学上的显著相互作用。
