Institute of Biotechnology, Shiraz University, Shiraz, Iran.
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
Bioorg Chem. 2021 May;110:104777. doi: 10.1016/j.bioorg.2021.104777. Epub 2021 Feb 24.
Despite the limitations of current methods in cancer treatment, the use of bioactive peptides can be as an alternative to treat today. Therefore, isolation and relative purification of bioactive peptides was carried out form Achillea eriophora using a Sep-Pak C18 SPE cartridge and Amicon® Ultra Centrifugal Filters. The presence of desired peptides was checked using RP-HPLC and confirmed using LC-MS. The results of anticancer assay showed that the peptide mixture inhibits the growth of MCF-7 cancerous cell line with the values of IC, GI, and LC equal to 18.73 ± 0.22, 7.52 ± 0.15, and 56.73 ± 0.18 µg/mL, respectively. It also showed DPPH radical scavenging activity and cupric-ion reducing power with the IC value of 5.095 ± 0.23 and 63.3 ± 0.44 µg/mL, respectively. Although flavonoids were present in the sample along with the peptides, their amount was trivial (18.097 ± 1.36 μg/mL). Nevertheless, the results of the LC-MS showed mass-to-charge ratios of 301.17, 261.22, and 243.25, which was a dipeptide or tripeptide in compression to enzyme-digested BSA as a standard. In addition, SEM analysis of the purified peptide mixture showed that it kills the MCF-7 cancerous cell line by creating pores in the membrane. Therefore, it might be valuable to these peptides sequenced and be studied for physicochemical properties. Animal and clinical studies could help its application in drug development.
尽管目前癌症治疗方法存在局限性，但生物活性肽的应用可以作为一种替代方法来治疗癌症。因此，使用 Sep-Pak C18 SPE 小柱和 Amicon® Ultra 离心过滤器从春黄菊中分离和相对纯化生物活性肽。使用 RP-HPLC 检查所需肽的存在，并使用 LC-MS 进行确认。抗癌测定的结果表明，肽混合物抑制 MCF-7 癌细胞系的生长，IC、GI 和 LC 值分别为 18.73±0.22、7.52±0.15 和 56.73±0.18 µg/mL。它还显示出 DPPH 自由基清除活性和铜离子还原能力，IC 值分别为 5.095±0.23 和 63.3±0.44 µg/mL。尽管样品中存在黄酮类化合物，但与肽一起，其含量微不足道（18.097±1.36 µg/mL）。尽管如此，LC-MS 的结果显示出 301.17、261.22 和 243.25 的质荷比，这与作为标准的酶解 BSA 相比是二肽或三肽。此外，纯化的肽混合物的 SEM 分析表明，它通过在膜中形成孔来杀死 MCF-7 癌细胞系。因此，对这些肽进行测序并研究其物理化学性质可能具有价值。动物和临床研究可以帮助其在药物开发中的应用。
