Taghizadeh Mohammad Sadegh, Niazi Ali, Retzl Bernhard, Gruber Christian W
Institute of Biotechnology, School of Agriculture, Shiraz University, Shiraz, Iran.
Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.
Heliyon. 2024 Nov 22;10(23):e40636. doi: 10.1016/j.heliyon.2024.e40636. eCollection 2024 Dec 15.
Currently, there is a growing preference for eco-friendly bioinsecticides over chemical insecticides due to their safety. Plant extracts have emerged as a promising solution for this purpose. Therefore, this study aimed to evaluate the insecticidal effectiveness of extract against two key pests of rose aphid () and pistachio psylla (). Significant compounds were identified using GC-MS and MALDI-TOF MS. Three bioassay methods were employed to assess the extract's insecticidal potential, and its cytotoxicity was tested on HEK293 cells. Results revealed that the highest insecticidal efficacy occurred at a concentration of 20 mg/mL after a 72 h exposure. The contact bioassay method displayed greater efficiency against than , while the oral bioassay demonstrated the highest efficiency against . The extract also acted as a feeding deterrent, with indices of 77.47 ± 7.98 % and 87.98 ± 3.84 % for and , respectively. Furthermore, the insecticidal potency of the extract was assessed, resulting in LC values of 3.58 mg/mL and 6.77 mg/mL for the contact bioassay, and 0.87 mg/mL and 0.61 mg/mL for the oral bioassay against and , respectively. Importantly, the extract showed no detrimental cytotoxic effects on the HEK293 cell line within the tested concentration range, indicating its potential safety as a bioinsecticide. Overall, these findings highlight the potential of extract as a promising candidate for further development in pest control.
目前，由于其安全性，人们越来越倾向于使用环保型生物杀虫剂而非化学杀虫剂。植物提取物已成为实现这一目的的一种有前景的解决方案。因此，本研究旨在评估提取物对玫瑰蚜虫（）和阿月浑子木虱（）这两种主要害虫的杀虫效果。使用气相色谱 - 质谱联用仪（GC - MS）和基质辅助激光解吸电离飞行时间质谱仪（MALDI - TOF MS）鉴定了重要化合物。采用三种生物测定方法评估提取物的杀虫潜力，并在人胚肾293（HEK293）细胞上测试其细胞毒性。结果显示，暴露72小时后，在浓度为20毫克/毫升时杀虫效果最佳。接触生物测定法对[具体害虫1]的效果比对[具体害虫2]的效果更好，而口服生物测定法对[具体害虫2]的效果最佳。该提取物还具有拒食作用，对[具体害虫1]和[具体害虫2]的拒食指数分别为77.47±7.98%和87.98±3.84%。此外，评估了提取物的杀虫效力，接触生物测定法对[具体害虫1]和[具体害虫2]的半数致死浓度（LC）值分别为3.58毫克/毫升和6.77毫克/毫升，口服生物测定法对[具体害虫1]和[具体害虫2]的LC值分别为0.87毫克/毫升和0.61毫克/毫升。重要的是，在测试浓度范围内，该提取物对HEK293细胞系未显示出有害的细胞毒性作用，表明其作为生物杀虫剂具有潜在的安全性。总体而言，这些发现突出了[提取物名称]提取物作为害虫防治进一步开发的有前景候选物的潜力。
