转录组分析为研究鱼类性腺中的长非编码 RNA 提供了新视角。
Transcriptome analysis provides insights into long noncoding RNAs in medaka gonads.
机构信息
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep;39:100842. doi: 10.1016/j.cbd.2021.100842. Epub 2021 Apr 29.
Long non-coding RNAs (lncRNAs) are gradually regarded as regulators in sex determination and gonad development of various animals. Medaka (Oryzias latipes) is an excellent reproductive research model with sex-determining genes. However, the regulation of gonadal lncRNAs on medaka reproductive development remains unknown. Here, 5317 lncRNAs were obtained from medaka ovary and testis by Illumina HiSeq4000, among which 177 lncRNAs were up-regulated and 120 lncRNAs were down-regulated in the testis compared to the ovary. In addition, 6904 cis-regulated target genes were predicted from 3099 lncRNAs. GO and KEGG enrichment analysis showed that these target genes were mainly involved in phosphorylation, metabolic, metabolism of xenobiotics by cytochrome P450, insulin secretion, and GnRH signaling pathways. Furthermore, six highly expressed lncRNAs were randomly selected to verify the sequencing data by quantitative real time PCR (qRT-PCR). Next, in situ hybridization revealed that one of the sex-biased lncRNA MSTRG.14827.1 was highly expressed in immature germ cells, indicating MSTRG.14827.1 may play a key role in gametogenesis. Taken together, this study provides emerging lncRNA libraries and opens new avenues for future investigation of lncRNAs in medaka.
长链非编码 RNA（lncRNAs）逐渐被视为各种动物性别决定和性腺发育的调控因子。青鳉（Oryzias latipes）是一种具有性别决定基因的优秀生殖研究模型。然而，lncRNAs 对青鳉生殖发育的调控作用仍不清楚。本研究通过 Illumina HiSeq4000 从青鳉卵巢和睾丸中获得了 5317 个 lncRNAs，其中 177 个在睾丸中上调，120 个在睾丸中下调。此外，从 3099 个 lncRNAs 中预测了 6904 个顺式调控靶基因。GO 和 KEGG 富集分析表明，这些靶基因主要参与磷酸化、代谢、细胞色素 P450 介导的外源物质代谢、胰岛素分泌和 GnRH 信号通路。进一步地，随机选择了 6 个高表达的 lncRNA 进行定量实时 PCR（qRT-PCR）验证测序数据。然后，原位杂交显示，6 个 sex-biased lncRNA 之一 MSTRG.14827.1 在未成熟生殖细胞中高度表达，表明 MSTRG.14827.1 可能在配子发生中发挥关键作用。总之，本研究提供了新兴的 lncRNA 文库，为未来青鳉 lncRNA 的研究开辟了新的途径。
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