Linc00641通过调节miR-429/Notch-1轴促进胃癌进展。
Linc00641 promotes the progression of gastric carcinoma by modulating the miR-429/Notch-1 axis.
作者信息
Hang Qun, Lu Jie, Zuo Lugen, Liu Mulin
机构信息
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, PR China.
Department of Operating Theatre, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, PR China.
出版信息
Aging (Albany NY). 2021 Mar 10;13(6):8497-8509. doi: 10.18632/aging.202661.
Linc00641 plays different roles in various types of human cancers. However, the function of linc00641 and its underlying mechanism of action in gastric cancer have not been fully elucidated. Therefore, the aim of our current study was to explore the molecular mechanism of linc00641 in gastric cancer. MTT assays, flow cytometry, wound healing assays, and Transwell invasion assays were utilized to measure cell viability, apoptosis, migration and invasion, respectively. Western blotting and RT-PCR assays were carried out to explore the mechanism of linc00641 in gastric cancer cells. We found that silencing linc00641 suppressed the viability and stimulated the apoptosis of gastric cancer cells, while linc00641 overexpression had the opposite effects. Moreover, linc00641 sponged the expression of miR-429 and subsequently upregulated Notch-1 expression in gastric cancer cells. We concluded that linc00641 promoted the malignant progression of gastric cancer by modulating the miR-429/Notch-1 axis.
Linc00641在各类人类癌症中发挥着不同作用。然而，Linc00641在胃癌中的功能及其潜在作用机制尚未完全阐明。因此，我们当前研究的目的是探究Linc00641在胃癌中的分子机制。采用MTT法、流式细胞术、伤口愈合试验和Transwell侵袭试验分别检测细胞活力、凋亡、迁移和侵袭情况。进行蛋白质免疫印迹法和逆转录-聚合酶链反应试验以探究Linc00641在胃癌细胞中的作用机制。我们发现，沉默Linc00641可抑制胃癌细胞活力并促进其凋亡，而Linc00641过表达则产生相反作用。此外，Linc00641可吸附miR-429的表达，进而上调胃癌细胞中Notch-1的表达。我们得出结论，Linc00641通过调节miR-429/Notch-1轴促进胃癌的恶性进展。
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