韩国国家干细胞银行。

Korea National Stem Cell Bank.

机构信息

Korea National Stem Cell Bank, South Korea; Division of Intractable Diseases Research, Department of Chronic Diseases Convergence Research, Korea National Institute of Health, Osong Health Technology Administration Complex 202, South Korea.

出版信息

Stem Cell Res. 2021 May;53:102270. doi: 10.1016/j.scr.2021.102270. Epub 2021 Feb 26.

DOI:10.1016/j.scr.2021.102270

PMID:33714852

Abstract

The Korea National Stem Cell Bank has been banking pluripotent stem cell (PSC) lines since 2012. Quality-controlled and ethically sourced cell lines were developed for distribution. Currently (as of 2020), among the 69 deposited lines, 4 research-grade human embryonic stem cell (hESC) lines and 19 induced pluripotent stem cell (iPSC) lines have been distributed. Good manufacturing practices (GMP)-compliant homozygous iPSC lines for regenerative medicine with homozygous HLA haplotypes that cover 51% of the Korean population have been deposited as well. To ensure the quality of the cell lines, we performed eighteen different quality tests on the identity, sterility, consistency, stability and safety of the cell lines. Regarding genetic stability, we are collecting SNPchip, WES, Methyl-seq, and RNA-seq data, which are open to the public.

摘要

韩国国家干细胞银行自 2012 年以来一直存储多能干细胞（PSC）系。已开发出经过质量控制和伦理来源的细胞系以供分发。目前（截至 2020 年），在已存入的 69 个细胞系中，已分发了 4 个研究级人类胚胎干细胞（hESC）系和 19 个诱导多能干细胞（iPSC）系。还存入了符合良好生产规范（GMP）的同种异体 HLA 单倍型覆盖 51%韩国人口的同种异体 iPSC 系，用于再生医学。为确保细胞系的质量，我们对细胞系的身份、无菌性、一致性、稳定性和安全性进行了十八项不同的质量测试。关于遗传稳定性，我们正在收集 SNPchip、WES、Methyl-seq 和 RNA-seq 数据，这些数据对公众开放。