Wang Quanxin, Wu Mingzhen, Xu Xiaoyang, Ding Chunmei, Luo Jianbin, Li Jianshu
College of Polymer Science and Engineering, Sichuan University, No. 24 South Section 1, Yihuan Road, Chengdu 610065, China.
College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, No. 16 South Section 4, Yihuan Road, Chengdu 610041, China.
ACS Biomater Sci Eng. 2021 Mar 8;7(3):852-861. doi: 10.1021/acsbiomaterials.9b01821. Epub 2020 Mar 20.
Hydroxyapatites (HAPs) are usually coated on the surface of an implant to improve the osseointegration with defect bone tissue. Besides, conducting polymers have the advantages of good conductivity, reasonable biocompatibility, and easy of modification, which endow them applicable to electrical stimulation therapy. However, it still remains a great challenge to fabricate hybrid coating combing HAP with conducting polymer on implant surface efficiently. In this work, phytic acid-doped polyaniline (PANI) were successfully synthesized on medical titanium (Ti) sheets. By virtue of the abundant anodic phosphoric groups of phytic acid, HAP nanocrystals were biomineralized on PANI. The PANI-HAP hybrid layer exhibits good cell compatibility with MC3T3 cells. More importantly, HAP nanocrystals and PANI operate synergistically on cell proliferation and osteogenesis through electrical stimulation. Alkaline phosphatase activity and extracellular calcium contents of cells on PANI-HAP display 3-fold and 2.6-fold increases, compared with bare Ti sheets, respectively. The valid integration of mineralization and electrical stimulation in this work renders an efficient strategy for implant coating, which might have potential applications in bone-related defects.
羟基磷灰石（HAPs）通常被涂覆在植入物表面，以改善与缺损骨组织的骨整合。此外，导电聚合物具有良好的导电性、合理的生物相容性和易于改性的优点，这使其适用于电刺激疗法。然而，在植入物表面高效制备结合HAP与导电聚合物的混合涂层仍然是一个巨大的挑战。在这项工作中，在医用钛（Ti）片上成功合成了植酸掺杂的聚苯胺（PANI）。借助植酸丰富的阳极磷酸基团，HAP纳米晶体在PANI上进行了生物矿化。PANI-HAP混合层与MC3T3细胞表现出良好的细胞相容性。更重要的是，HAP纳米晶体和PANI通过电刺激在细胞增殖和成骨过程中协同作用。与裸Ti片相比，PANI-HAP上细胞的碱性磷酸酶活性和细胞外钙含量分别增加了3倍和2.6倍。这项工作中矿化和电刺激的有效整合为植入物涂层提供了一种有效策略，这可能在骨相关缺损中具有潜在应用。
