College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.
Biomater Sci. 2019 Nov 1;7(11):4730-4737. doi: 10.1039/c9bm01203f. Epub 2019 Sep 9.
Electrical stimulation (ES) exhibits a positive role in promoting the cell activity of osteoblasts. Conducting polymers have the advantages of biocompatibility, good environmental stability and easy synthesis, which have been widely used as charge carriers for electrical stimulation; moreover, considering clinical applications, biomaterial-related infection is an important issue that needs to be solved. Thus, conducting polymers with both antibacterial and osteogenic properties are highly demanded for effect repair. However, it remains a challenge to combine these two characteristics efficiently in a simple way. Herein, an Ag-loaded poly(amide-amine) dendrimer was prepared by a simple chemical reduction procedure, which acted as a dopant for the polymerization of polyaniline (PANI) on biomedical titanium (Ti) sheets. The obtained PANI coating showed outstanding antibacterial properties against Gram-negative (E. coli) and Gram-positive (S. aureus) microbes with a 1000-fold increase when compared with that of pure Ti. In addition, note that the polymer coating together with ES facilitated the proliferation and differentiation of MC3T3. The alkaline phosphatase (ALP) activity and intracellular calcium content of the cells showed a 19.09% and 24.02% increase, respectively, when compared with the case of electrically stimulated Ti after 12 days. Moreover, the existence of PAMAM facilitated mineralization. The strategy developed herein is simple and can be easily manipulated, which shows potential applications in the coating of implants for hard tissue repair.
电刺激（ES）在促进成骨细胞的细胞活性方面表现出积极作用。导电聚合物具有生物相容性、良好的环境稳定性和易于合成的优点，已被广泛用作电刺激的电荷载体；此外，考虑到临床应用，与生物材料相关的感染是一个需要解决的重要问题。因此，具有抗菌和成骨特性的导电聚合物非常需要用于修复效果。然而，以简单的方式有效地结合这两个特性仍然是一个挑战。在此，通过简单的化学还原法制备了负载银的聚（酰胺-胺）树枝状大分子，它作为掺杂剂用于在生物医学钛（Ti）片上聚合聚苯胺（PANI）。所得的 PANI 涂层对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）微生物表现出出色的抗菌性能，与纯 Ti 相比增加了 1000 倍。此外，值得注意的是，聚合物涂层与 ES 一起促进了 MC3T3 的增殖和分化。与电刺激 Ti 后 12 天相比，细胞的碱性磷酸酶（ALP）活性和细胞内钙含量分别增加了 19.09%和 24.02%。此外，PAMAM 的存在促进了矿化。本研究开发的策略简单且易于操作，在硬组织修复植入物涂层方面具有潜在的应用前景。
