Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), Institute of Bio-molecular and Biomedical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, P.R. China.
Biomater Sci. 2022 Jun 14;10(12):3054-3061. doi: 10.1039/d2bm00445c.
DNA-based materials exhibit great potential in biomedical applications due to their excellent sequence programmability and unique functional designability. Rolling circle amplification (RCA) is an efficient isothermal enzymatic amplification strategy to produce ultralong single-stranded DNA with customized functional moieties. The rational design of RCA templates and the introduction of other functional components enable RCA-based DNA materials with structural dynamic responsiveness and diverse biological functions, facilitating the development of DNA-based materials in biomedical applications. In this review, the principle and synthetic methods of RCA and the recent progress of RCA-based DNA nanostructures for therapeutics and bioimaging are summarized and discussed. The future challenges and opportunities for RCA-based DNA nanostructures are discussed at the end of the study. We envision that the development of RCA-based DNA nanostructures will provide more possibilities for precision medicine.
基于 DNA 的材料由于其出色的序列可编程性和独特的功能设计能力，在生物医学应用中具有巨大的潜力。滚环扩增（RCA）是一种有效的等温酶促扩增策略，可用于生产具有定制功能部分的超长单链 DNA。通过合理设计 RCA 模板和引入其他功能组件，能够使基于 RCA 的 DNA 材料具有结构动态响应性和多种生物学功能，从而促进了基于 DNA 的材料在生物医学应用中的发展。在本综述中，总结和讨论了 RCA 的原理和合成方法以及基于 RCA 的 DNA 纳米结构在治疗和生物成像方面的最新进展。最后讨论了基于 RCA 的 DNA 纳米结构未来的挑战和机遇。我们设想，基于 RCA 的 DNA 纳米结构的发展将为精准医疗提供更多的可能性。
