Dufour C, Dandrifosse G, Forget P, Vermesse F, Romain N, Lepoint P
Department of Pediatrics, University of Liège, Belgium.
Gastroenterology. 1988 Jul;95(1):112-6. doi: 10.1016/0016-5085(88)90298-3.
In the present study, we aimed to induce precocious intestinal maturation in neonatal rats by the oral administration of polyamines. Groups of 5 rats received either saline, spermidine (10 mumol daily), or spermine (6 mumol daily) orally on the 12th, 13th, and 14th postnatal days. The rats were killed on the 15th postnatal day. After the small bowel was removed, a 1-cm distal ileal segment was removed for histologic examination and the remaining small bowel tissue was homogenized for further biochemical analysis. Polyamine administration was shown to induce structural and biochemical mucosal changes characteristic of postnatal maturation. Lactase, sucrase, and maltase specific activities (micromoles of substrate hydrolyzed per minute per gram of protein) were 80 +/- 10, 10 +/- 3, and 116 +/- 19 for the saline-treated rats; 51 +/- 7, 34 +/- 2, and 315 +/- 37 for the spermidine-treated rats; and 25 +/- 2, 46 +/- 5, and 419 +/- 63 for the spermine-treated rats, respectively. Similar results were obtained with rats, first treated with spermine (6 mumol) on the 7th postnatal day, receiving spermine (6 mumol) daily as described above and killed on the 10th postnatal day. Dose-response experiments performed as reported above in rats whose treatment began on the 12th postnatal day showed that the maturational effects of orally administered spermine are dose-dependent.
在本研究中，我们旨在通过口服多胺诱导新生大鼠肠道早熟。将5只大鼠分为一组，在出生后第12、13和14天分别口服生理盐水、亚精胺（每日10 μmol）或精胺（每日6 μmol）。在出生后第15天处死大鼠。取出小肠后，取1 cm远侧回肠段进行组织学检查，其余小肠组织匀浆用于进一步生化分析。结果显示，给予多胺可诱导出产后成熟所特有的结构和生化黏膜变化。生理盐水处理组大鼠的乳糖酶、蔗糖酶和麦芽糖酶比活性（每克蛋白质每分钟水解底物的微摩尔数）分别为80±10、10±3和116±19；亚精胺处理组大鼠分别为51±7、34±2和315±37；精胺处理组大鼠分别为25±2、46±5和419±63。对出生后第7天首次给予精胺（6 μmol）、然后按上述方法每日给予精胺（6 μmol）并在出生后第10天处死的大鼠，也得到了类似结果。在出生后第12天开始治疗的大鼠中按上述方法进行的剂量反应实验表明，口服精胺的成熟作用具有剂量依赖性。
