基于现场和实验贻贝样本的麻痹性贝类毒素新代谢物的拟议生物转化途径。

Proposed Biotransformation Pathways for New Metabolites of Paralytic Shellfish Toxins Based on Field and Experimental Mussel Samples.

机构信息

College of Environmental Science and Engineering, Ocean University of China , Qingdao, Shandong 266100, People's Republic of China.

Key Laboratory of Marine Environment and Ecology, Ocean University of China, Ministry of Education , Qingdao, Shandong 266100, People's Republic of China.

出版信息

J Agric Food Chem. 2017 Jul 12;65(27):5494-5502. doi: 10.1021/acs.jafc.7b02101. Epub 2017 Jun 27.

DOI:10.1021/acs.jafc.7b02101

PMID:28616979

Abstract

A seafood poisoning event occurred in Qinhuangdao, China, in April 2016. Subsequently, the causative mussels (Mytilus galloprovincialis) were harvested and analyzed to reveal a high concentration [∼10 758 μg of saxitoxin (STX) equiv kg] of paralytic shellfish toxins (PSTs), including gonyautoxin (GTX)1/4 and GTX2/3, as well as new metabolites 11-hydroxy-STX (M2), 11,11-dihydroxy-STX (M4), open-ring 11,11-dihydroxy-STX (M6), 11-hydroxy-neosaxitoxin (NEO) (M8), and 11,11-dihydroxy-NEO (M10). To understand the origin and biotransformation pathways of these new metabolites, uncontaminated mussels (M. galloprovincialis) were fed with either of two Alexandrium tamarense strains (ATHK and TIO108) under laboratory conditions. Similar PST metabolites were also detected in mussels from both feeding experiments. Results supposed that 11-hydroxy-C2 toxin (M1) and 11,11-dihydroxy-C2 (M3) are transformed from C2, while 11-hydroxy-C4 toxin (M7) and 11,11-dihydroxy-C4 (M9) are converted from C4. In addition, the metabolites M2, M4, and M6 appear to be products of GTX2/3, and the metabolites M8 and M10 are likely derived from GTX1/4.

摘要

2016 年 4 月，中国秦皇岛发生一起海产品中毒事件。随后，对中毒贻贝类（马氏珠母贝）进行了采集和分析，结果显示麻痹性贝类毒素（PSTs）浓度较高（[∼10758μg 石房蛤毒素（STX）当量/kg]），包括膝沟藻毒素（GTX）1/4 和 GTX2/3，以及新代谢物 11-羟基 STX（M2）、11,11-二羟基 STX（M4）、开环 11,11-二羟基 STX（M6）、11-羟基neosaxitoxin（NEO）（M8）和 11,11-二羟基 NEO（M10）。为了了解这些新代谢物的来源和生物转化途径，在实验室条件下，用两种亚历山大藻（ATHK 和 TIO108）喂养未受污染的贻贝（马氏珠母贝）。在这两种喂养实验的贻贝中也检测到了类似的 PST 代谢物。结果表明，11-羟基-C2 毒素（M1）和 11,11-二羟基-C2（M3）是由 C2 转化而来，而 11-羟基-C4 毒素（M7）和 11,11-二羟基-C4（M9）是由 C4 转化而来。此外，代谢物 M2、M4 和 M6 似乎是 GTX2/3 的产物，代谢物 M8 和 M10 可能来源于 GTX1/4。