Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Cell and Molecular Biology, Faculty of Science, Kosar University of Bojnord, Bojnord, Iran.
J Ethnopharmacol. 2021 Jun 12;273:114023. doi: 10.1016/j.jep.2021.114023. Epub 2021 Mar 11.
Parkinson's disease (PD) is a neurodegenerative disorder associated with oxidative stress-induced neuronal damage and death. In European and Persian Traditional Medicine, aerial parts (leaves, stems, and flowers) of Lavandula stoechas L. have been widely used for treating neurodegenerative disorders including PD.
Herein, the protective effects of L. stoechas methanol extract were investigated on 6-hydroxydopamine (6-OHDA)-induced cytotoxicity and oxidative damage in PC12 cells.
The cells were pretreated with a standardized L. stoechas methanol extract (2.5-20 μg/mL) for 24 h and exposed to 6-OHDA (200 μM) thereafter. The cell viability percentage was determined by AlamarBlue test. Intracellular reactive oxygen species (ROS) production was determined by a fluorimetric method using 2',7'-dichlorodihydrofluorescein diacetate and cellular apoptosis was assessed by the fluorescent probe propidium iodide test. Finally, the expression of proteins involved in apoptosis pathway (Phospho SAPK/JNK, SAPK/JNK, p44/42 MAPK (ERK1/2) and Poly ADP ribose polymerase (PARP)) was measured via Western blot analysis.
Treatment of PC12 cells with 6-OHDA could significantly increase cytotoxicity, ROS level, and cell apoptosis. Pretreatment of PC12 cells with the extract could significantly decrease 6-OHDA cytotoxicity, ROS production, (2.5 and 5 μg/mL) and cell apoptosis (5 μg/mL). Western blot analysis showed that 6-OHDA exposure could increase the expression of proteins involved in apoptosis signaling, while pretreatment with L. stoechas (5 μg/mL) reduced apoptotic proteins.
The present study demonstrated that L. stoechas, which has been traditionally used in Persian Medicine for treating CNS diseases, is a valuable source of active compounds with neuroprotective, anti-oxidant, and anti-apoptotic activity.
帕金森病（PD）是一种与氧化应激诱导的神经元损伤和死亡有关的神经退行性疾病。在欧洲和波斯传统医学中，薰衣草（Lavandula stoechas L.）的地上部分（叶、茎和花）已被广泛用于治疗包括 PD 在内的神经退行性疾病。
本研究旨在探讨薰衣草甲醇提取物对 6-羟多巴胺（6-OHDA）诱导的 PC12 细胞毒性和氧化损伤的保护作用。
细胞用标准化薰衣草甲醇提取物（2.5-20μg/mL）预处理 24 小时，然后暴露于 6-OHDA（200μM）。通过 AlamarBlue 试验测定细胞存活率百分比。通过 2'，7'-二氯二氢荧光素二乙酸酯的荧光法测定细胞内活性氧（ROS）的产生，并用碘化丙啶荧光探针测定细胞凋亡。最后，通过 Western blot 分析测定参与细胞凋亡途径的蛋白质（磷酸 SAPK/JNK、SAPK/JNK、p44/42 MAPK（ERK1/2）和多聚 ADP 核糖聚合酶（PARP））的表达。
用 6-OHDA 处理 PC12 细胞可显著增加细胞毒性、ROS 水平和细胞凋亡。用提取物预处理 PC12 细胞可显著降低 6-OHDA 的细胞毒性、ROS 产生（2.5 和 5μg/mL）和细胞凋亡（5μg/mL）。Western blot 分析表明，6-OHDA 暴露可增加参与细胞凋亡信号的蛋白质的表达，而用薰衣草（5μg/mL）预处理可减少凋亡蛋白。
本研究表明，薰衣草在波斯医学中被传统用于治疗中枢神经系统疾病，是具有神经保护、抗氧化和抗凋亡活性的有价值的活性化合物来源。
