Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Cell and Molecular Biology, Faculty of Science, Kosar University of Bojnord, Bojnord, Iran.
Mol Biol Rep. 2020 Apr;47(4):2437-2445. doi: 10.1007/s11033-020-05284-y. Epub 2020 Mar 12.
Cinnamon (Cinnamomum verum and C. cassia) is a medicinal plant, widely-used as a culinary spice. It possesses various therapeutic effects and can slow down the progression of neurological disorders impressively. In this article, the effects of hydro-alcohol extract and essential oil of C. verum and C. cassia and its main bioactive component cinnamaldehyde, has been examined on 6-OHDA-exposed PC12 cells as an in vitro model of Parkinson's disease. The cytotoxicity and cell apoptosis has been induced by 6-OHDA in PC12 cells. The protective effect was determined by measuring cell viability, the amount of reactive oxygen species (ROS), and apoptosis. Cell viability and apoptosis were assessed using resazurin assay, flow cytometry of propidium iodide (PI) stained cells, and western blot analysis. 6-OHDA resulted in the death and apoptosis of cells while, pretreatment with the extract and essential oil of C. verum and C. cassia at 20 µg/ml and cinnamaldehyde at 5 and 10 µM for 24 h could significantly increase the viability (p < 0.001), and decrease ROS content (p < 0.05). Pretreatment with the extracts increased survivin and decreased cyt-c whereas, pretreatment with the essential oil decreased cyt-c, increased survivin, and reduced P-p44/42/p44/42 levels to a level near that of the related control. The extract and essential oil of C. verum and C. cassia can be effective against 6-OHDA cytotoxicity. It is suggested that, the synergistic effects of cinnamaldehyde and other components of extract and essential oil promote cinnamon's medicinal properties.
肉桂（Cinnamomum verum 和 C. cassia）是一种药用植物，广泛用作烹饪香料。它具有多种治疗作用，可以显著减缓神经退行性疾病的进展。在本文中，研究了 C. verum 和 C. cassia 的水醇提取物和精油及其主要生物活性成分肉桂醛对 6-OHDA 暴露的 PC12 细胞的影响，PC12 细胞作为帕金森病的体外模型。6-OHDA 诱导 PC12 细胞产生细胞毒性和细胞凋亡。通过测量细胞活力、活性氧 (ROS) 量和细胞凋亡来确定保护作用。使用 Resazurin 测定法、碘化丙啶 (PI) 染色细胞的流式细胞术和 Western blot 分析评估细胞活力和细胞凋亡。6-OHDA 导致细胞死亡和凋亡，而用提取物和精油预处理 20 µg/ml 的 C. verum 和 C. cassia 以及 5 和 10 µM 的肉桂醛 24 h 可显著增加细胞活力（p<0.001），并降低 ROS 含量（p<0.05）。提取物预处理可增加 survivin 并降低 cyt-c，而精油预处理可降低 cyt-c，增加 survivin，并将 P-p44/42/p44/42 水平降低至接近相关对照的水平。C. verum 和 C. cassia 的提取物和精油可有效抵抗 6-OHDA 的细胞毒性。建议肉桂醛和提取物及精油中其他成分的协同作用促进了肉桂的药用特性。
