Fa Shixin, Zhao Yan
Department of Chemistry, Iowa State University, Ames, IA, 50011-3111, USA.
Chemistry. 2018 Jan 2;24(1):150-158. doi: 10.1002/chem.201703760. Epub 2017 Dec 4.
Sequence-specific recognition of peptides is of enormous importance to many chemical and biological applications, but has been difficult to achieve due to the minute differences in the side chains of amino acids. Acidic peptides are known to play important roles in cell growth and gene expression. In this work, we report molecularly imprinted micelles coded with molecular recognition information for the acidic and hydrophobic side chains of acidic peptides. The imprinted receptors could distinguish acidic amino acids from other polar and nonpolar amino acids, with dissociation constants of tens of nanomolar for biologically active peptides containing up to 18 amino acids.
肽的序列特异性识别对许多化学和生物学应用极为重要，但由于氨基酸侧链的微小差异，一直难以实现。已知酸性肽在细胞生长和基因表达中发挥重要作用。在这项工作中，我们报道了用酸性肽的酸性和疏水侧链的分子识别信息编码的分子印迹胶束。印迹受体能够区分酸性氨基酸与其他极性和非极性氨基酸，对于含有多达18个氨基酸的生物活性肽，其解离常数为几十纳摩尔。
