Zhang Xin, Yang Shu, Chen Weijie, Li Yansong, Wei Yuping, Luo Aiqin
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China.
School of Life Science, Beijing Institute of Technology, No.5 Zhongguancun South Street, Beijing 100081, China.
Polymers (Basel). 2019 Jul 19;11(7):1210. doi: 10.3390/polym11071210.
In this study, magnetic fluorescence molecularly imprinted polymers were fabricated and used for the selective separation and fluorescence sensing of trace bisphenol A (BPA) in environmental water samples. The carboxyl-functionalized FeO magnetic nanoparticles were conjugated with mercaptoethylamine-capped Mn doped ZnS quantum dots to prepare magnetic FeO and ZnS quantum dot nanoparticles (FeO/ZnS NPs). Additionally, molecular imprinting on the FeO/ZnS NPs was employed to synthesize core-shell molecularly imprinted polymers. The resulting nanoparticles were well characterized using transmission electron microscopy, Fourier transform infrared spectra, vibrating sample magnetometer and fluorescence spectra, and the adsorption behavior was investigated. Binding experiments showed that the molecularly imprinted FeO/ZnS NPs (FeO/ZnS@MIPs) exhibited rapid fluorescent and magnetic responses, and high selectivity and sensitivity for the detection of bisphenol A (BPA). The maximum adsorption capacity of FeO/ZnS@MIPs was 50.92 mg·g with an imprinting factor of 11.19. Under optimal conditions, the constructed fluorescence magnetic molecularly imprinted polymers presented good linearity from 0 to 80 ng mL with a detection limit of 0.3626 ng mL for BPA. Moreover, the proposed fluorescence magnetic polymers were successfully applied to on-site magnetic separation and real-time fluorescence analysis of target molecule in real samples.
在本研究中，制备了磁性荧光分子印迹聚合物，并将其用于环境水样中痕量双酚A（BPA）的选择性分离和荧光传感。将羧基功能化的FeO磁性纳米颗粒与巯基乙胺封端的Mn掺杂ZnS量子点共轭，制备磁性FeO和ZnS量子点纳米颗粒（FeO/ZnS NPs）。此外，采用在FeO/ZnS NPs上进行分子印迹的方法合成核壳型分子印迹聚合物。使用透射电子显微镜、傅里叶变换红外光谱、振动样品磁强计和荧光光谱对所得纳米颗粒进行了充分表征，并研究了其吸附行为。结合实验表明，分子印迹的FeO/ZnS NPs（FeO/ZnS@MIPs）对双酚A（BPA）的检测表现出快速的荧光和磁响应，以及高选择性和灵敏度。FeO/ZnS@MIPs的最大吸附容量为50.92 mg·g，印迹因子为11.19。在最佳条件下，构建的荧光磁性分子印迹聚合物在0至80 ng mL范围内呈现良好的线性关系，对BPA的检测限为0.3626 ng mL。此外，所提出的荧光磁性聚合物成功应用于实际样品中目标分子的现场磁分离和实时荧光分析。
