Hu Jingyun, Cai Ming, Shang Qinghui, Li Zhaorun, Feng Yu, Liu Beibei, Xue Xiangli, Lou Shujie
Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China.
College of Rehabilitation Sciences, Shanghai University of Medicine & Health Sciences, Shanghai, China.
Front Physiol. 2021 Feb 25;12:629914. doi: 10.3389/fphys.2021.629914. eCollection 2021.
High-intensity interval training (HIIT) is reported to be beneficial to brain-derived neurotrophic factor (BDNF) biosynthesis. A key element in this may be the existence of lactate, the most obvious metabolic product of exercise. , this study investigated the effects of a 6-week HIIT on the peripheral and central lactate changes, mitochondrial quality control system, mitochondrial function and BDNF expression in mouse hippocampus. , primary cultured mice hippocampal cells were used to investigate the role and the underlying mechanisms of lactate in promoting mitochondrial function during HIIT. studies, we firstly reported that HIIT can potentiate mitochondrial function [boost some of the mitochondrial oxidative phosphorylation (OXPHOS) genes expression and ATP production], stimulate BDNF expression in mouse hippocampus along with regulating the mitochondrial quality control system in terms of promoting mitochondrial fusion and biogenesis, and suppressing mitochondrial fission. In parallel to this, the peripheral and central lactate levels elevated immediately after the training. study, our results revealed that lactate was in charge of regulating mitochondrial quality control system for mitochondrial function and thus may contribute to BDNF expression. In conclusion, our study provided the mitochondrial mechanisms of HIIT enhancing brain function, and that lactate itself can mediate the HIIT effect on mitochondrial quality control system in the hippocampus.
据报道，高强度间歇训练（HIIT）对脑源性神经营养因子（BDNF）的生物合成有益。其中的一个关键因素可能是乳酸的存在，它是运动最明显的代谢产物。因此，本研究调查了为期6周的HIIT对小鼠外周和中枢乳酸变化、线粒体质量控制系统、线粒体功能以及海马体中BDNF表达的影响。此外，使用原代培养的小鼠海马体细胞来研究乳酸在HIIT期间促进线粒体功能中的作用及其潜在机制。在研究中，我们首次报道HIIT可以增强线粒体功能[提高一些线粒体氧化磷酸化（OXPHOS）基因的表达和ATP生成]，刺激小鼠海马体中的BDNF表达，同时在促进线粒体融合和生物发生以及抑制线粒体分裂方面调节线粒体质量控制系统。与此同时，训练后外周和中枢乳酸水平立即升高。在本研究中，我们的结果表明乳酸负责调节线粒体质量控制系统以维持线粒体功能，因此可能有助于BDNF表达。总之，我们的研究提供了HIIT增强脑功能的线粒体机制，并且乳酸本身可以介导HIIT对海马体线粒体质量控制系统的影响。
