Pereira Felicidade Mota, Ramos Pablo Ivan Pereira, Lirio Monique, Mercês Atta Ajax, Silva de Oliveira Isabela, Nascimento Fabio Carneiro Vosqui, Silva Marcelo Costa, Galvão-Castro Bernardo, Rios Grassi Maria Fernanda
Laboratório Avançado de Saúde Pública, Fundação Oswaldo Cruz, Salvador, Brazil.
Laboratório Central de Saúde Pública Prof. Gonçalo Moniz - Secretaria da Saúde do Estado da Bahia, Salvador, Brazil.
Front Microbiol. 2021 Feb 26;12:632695. doi: 10.3389/fmicb.2021.632695. eCollection 2021.
Co-infection between the human T-cell lymphotropic virus (HTLV) and the hepatitis C virus (HCV) can modify the natural history of HCV infection. The aim of this study was to describe the inflammatory cytokines and IL-10 network in patients co-infected with HTLV and HCV viruses in Bahia, Brazil.
Samples from 31 HTLV/HCV co-infected individuals and 27 HCV monoinfected individuals were evaluated. IFN-γ, TNF-α, IL-10, IL-8, and IL-1 cytokines were quantified by ELISA. Clinical, laboratory data were obtained from patient records. Serum levels of the cytokines were log-transformed and data mining was performed using Z-score statistics and correlation analysis.
Co-infected individuals presented a tendency toward higher production of INF-γ compared to the HCV monoinfected group. Regarding cytokine pairs, there was a positive correlation (-value < 0.05) between IL-1 and IL-8 in the HTLV/HCV co-infected group and uninfected controls, and two correlations in the HCV mono-infected group IL-8 - IL10 and IL- INF-γ - IL-10 pairs. There was no significant difference between the groups for the other parameters analyzed.
The results presented herein indicated that HTLV/HCV co-infection was associated with a trend in IFN-γ production while HCV-infected individuals presented a positive correlation with both inflammatory cytokines (IL-8 and IFN-γ) and the regulatory cytokine IL-10.
人类嗜T细胞病毒（HTLV）与丙型肝炎病毒（HCV）的合并感染可改变HCV感染的自然病程。本研究旨在描述巴西巴伊亚州HTLV和HCV病毒合并感染患者的炎性细胞因子和白细胞介素-10网络。
对31例HTLV/HCV合并感染个体和27例HCV单一感染个体的样本进行评估。采用酶联免疫吸附测定法（ELISA）对干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-8（IL-8）和白细胞介素-1（IL-1）细胞因子进行定量分析。临床和实验室数据来自患者记录。对细胞因子的血清水平进行对数转换，并使用Z评分统计和相关性分析进行数据挖掘。
与HCV单一感染组相比，合并感染个体呈现出更高水平的INF-γ产生趋势。关于细胞因子对，在HTLV/HCV合并感染组和未感染对照组中，IL-1和IL-8之间存在正相关（P值<0.05），在HCV单一感染组中，IL-8与IL-10以及IL-INF-γ与IL-10对之间存在两种相关性。分析的其他参数在两组之间没有显著差异。
本文给出的结果表明，HTLV/HCV合并感染与IFN-γ产生趋势有关，而HCV感染个体与炎性细胞因子（IL-8和IFN-γ）和调节性细胞因子IL-10均呈正相关。
