Seersholm Frederik V, Hansen Kasper Lykke, Heydenrych Matthew, Hansen Anders J, Bunce Michael, Allentoft Morten E
Trace and Environmental DNA (TrEnD) Laboratory School of Molecular and Life Sciences Curtin University Bentley WA Australia.
Section for GeoGenetics GLOBE Institute University of Copenhagen Copenhagen Denmark.
Ecol Evol. 2021 Feb 5;11(5):2064-2071. doi: 10.1002/ece3.7162. eCollection 2021 Mar.
The Lund collection is one of the oldest subfossil collections in the world. The vast assemblage of subfossils was collected in the 1830s and 1840s by Peter Wilhelm Lund in Lagoa Santa, Brazil, and was shipped to Copenhagen in 1848, where it was stored in various locations around the city with little attention for the future preservation of the collection. So far, successful genetic research on the material collected by Lund has been limited to two samples of human petrous bone. However, less is known about the preservation conditions of the vast amounts of small and fragmentary bones stored in the collection. To address this, we studied ancient DNA from bulk bone samples of approximately 100 bone fragments from the P.W. Lund collection from boxes with varying degrees of physical preservation conditions. Using bulk bone metabarcoding, we found a high species diversity in all samples. In total, we identified 17 species, representing 11 mammals, two birds, one fish, and three frogs. Of these, two species are new to the collection. Collectively, these results exhibit the potential of future genetic studies on the famous P.W. Lund collection and suggest that the effects of poor storage conditions are probably negligible compared with the long-term in situ degradation that specimens undergo before excavation.
隆德化石收藏是世界上最古老的亚化石收藏之一。这些大量的亚化石是19世纪30年代和40年代由彼得·威廉·隆德在巴西拉戈阿桑塔收集的，并于1848年运往哥本哈根，存放在该市各地，对该收藏的未来保存几乎没有给予关注。到目前为止，对隆德收集的材料进行的成功基因研究仅限于两个人类颞骨样本。然而，对于收藏中大量小而零碎的骨头的保存状况了解较少。为了解决这个问题，我们研究了来自P.W.隆德收藏中约100块骨碎片的批量骨样本中的古DNA，这些样本来自保存状况不同的盒子。通过批量骨元条形码技术，我们在所有样本中发现了高度的物种多样性。我们总共鉴定出17个物种，包括11种哺乳动物、2种鸟类、1种鱼类和3种青蛙。其中，有两个物种是该收藏中的新物种。总体而言，这些结果展示了未来对著名的P.W.隆德收藏进行基因研究的潜力，并表明与标本在挖掘前经历的长期原地降解相比，储存条件不佳的影响可能微不足道。
