Wang Ruihua, Han Taili, Sun Jifeng, Xu Ligong, Fan Jingjing, Cao Hui, Liu Chunxiang
Biological and Agricultural College, Weifang University, Weifang, China.
Vegetable Research Institute, Weifang Academy of Agricultural Sciences, Weifang, China.
PeerJ. 2021 Mar 5;9:e10934. doi: 10.7717/peerj.10934. eCollection 2021.
Ovate family proteins (OFPs) are a class of proteins with a conserved OVATE domain that contains approximately 70 amino acid residues. OFP proteins are plant-specific transcription factors that participate in regulating plant growth and development and are widely distributed in many plants. Little is known about OFPs in to date. We identified 29 genes in and found that they were unevenly distributed on 10 chromosomes. Intron gain events may have occurred during the structural evolution of paralogues. Syntenic analysis verified genome triplication, and whole genome duplication likely contributed to the expansion of the gene family. All genes had light responsive- and phytohormone-related cis-acting elements. Expression analysis from RNA-Seq data indicated that there were obvious changes in the expression levels of six genes in the hybrid, which may contribute to the formation of heterosis. Finally, we found that the paralogous genes had different expression patterns among the hybrid and its parents. These results provide the theoretical basis for the further analysis of the biological functions of genes across the species.
卵形家族蛋白（OFPs）是一类具有保守卵形结构域的蛋白质，该结构域包含约70个氨基酸残基。OFP蛋白是植物特有的转录因子，参与调节植物生长发育，广泛分布于多种植物中。迄今为止，关于OFP在[具体物种]中的情况知之甚少。我们在[具体物种]中鉴定出29个[基因名称]基因，发现它们在10条染色体上分布不均。在[基因名称]旁系同源基因的结构进化过程中可能发生了内含子获得事件。共线性分析证实了[具体物种]基因组三倍化，全基因组复制可能促成了[基因名称]基因家族的扩张。所有[基因名称]基因都具有光响应和植物激素相关的顺式作用元件。基于RNA-Seq数据的表达分析表明，在[具体杂交组合]杂种中六个[基因名称]基因的表达水平有明显变化，这可能有助于杂种优势的形成。最后，我们发现旁系同源基因在杂种及其亲本中的表达模式不同。这些结果为进一步分析[具体物种]中[基因名称]基因的生物学功能提供了理论依据。
