Developmental Haematopoiesis Group, Faculty of Biology, Medicine and Health, the University of Manchester, Manchester M13 9PT, UK.
Stem Cell Process Development, Adaptimmune Ltd., 60 Jubilee Avenue Milton Park, Abingdon, Oxfordshire OX14 4RX, UK.
STAR Protoc. 2021 Mar 2;2(1):100367. doi: 10.1016/j.xpro.2021.100367. eCollection 2021 Mar 19.
Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed. For complete details on the use and execution of this protocol, please refer to Garcia-Alegria et al. (2018).
由于伦理原因和胚胎获取受限,人类胚胎发生过程中血液祖细胞的出现知之甚少。使用人类胚胎干细胞 (hESC) 作为模型系统为解析人类血细胞形成提供了独特的机会。在这里,我们描述了一种通过胚状体将 hESC 分化为造血内皮细胞并随后分化为血液祖细胞的方案。该方案依赖于胚状体的形成,如果操作不仔细,这将很棘手。有关此方案的使用和执行的完整详细信息,请参阅 Garcia-Alegria 等人。(2018)。