Department of Chemistry, Hacettepe Unıversity, Beytepe, Ankara, Turkey.
J Mol Recognit. 2021 Aug;34(8):e2895. doi: 10.1002/jmr.2895. Epub 2021 Mar 14.
Molecularly imprinted thin membranes for selective removal of tetracycline from real water samples were prepared using the radiation-induced polymerization method. The chemical and physical characterization of the membranes was conducted by FTIR, XPS, AFM and SEM analyses. The effect of the template on the size and the size distribution of the pores in the membrane structure was investigated by positron annihilation lifetime spectroscopy (PALS) experiments. The presence of the template molecule causes the formation of larger cavities, which have a strong correlation to the molecular size of the template molecule. The density of the free volume holes in the imprinted network shows a significant increase due to the presence of the template molecule. The binding performances of the membranes were tested against various factors such as pH, time and initial concentration of template molecules. The specific selectivity of the membranes was investigated by cross-reactivity experiments. The binding capacity of the imprinted membranes was obtained at 14.5 μmol g , with the highest imprinting factor (2.84) for tetracycline. The imprinting factor was obtained at 1.44, 1.25 and 1.57 for oxytetracycline hydrochloride, doxycycline hyclate and chlortetracycline, respectively. The binding capability of the imprinted membranes in real water samples was promising for the application of the membranes as a filter material for removal of tetracycline with high binding capacity. Tetracycline binding percentage of the membranes was determined at 92.4, 81.1., 75.0 and 68.7 for ultra-pure water, tap water and natural water samples collected from different sources, respectively.
采用辐射引发聚合方法制备了用于从实际水样中选择性去除四环素的分子印迹薄膜。通过傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）、原子力显微镜（AFM）和扫描电子显微镜（SEM）分析对膜进行了化学和物理表征。通过正电子湮没寿命谱（PALS）实验研究了模板对膜结构中孔的大小和尺寸分布的影响。模板分子的存在导致形成较大的空腔，这与模板分子的分子大小有很强的相关性。由于模板分子的存在，印迹网络中自由体积孔的密度显著增加。通过交叉反应实验研究了膜对各种因素（如 pH 值、时间和模板分子的初始浓度）的结合性能。通过研究交叉反应性实验研究了膜的特异性选择性。印迹膜的结合容量在 14.5 μmol/g 时达到最大值，四环素的最高印迹因子（2.84）。盐酸土霉素、盐酸强力霉素和金霉素的印迹因子分别为 1.44、1.25 和 1.57。印迹膜在实际水样中的结合能力为将其作为具有高结合能力的四环素去除用过滤材料的应用提供了广阔的前景。在超纯水、自来水和来自不同来源的天然水样中，膜对四环素的结合百分比分别为 92.4%、81.1%、75.0%和 68.7%。
