The Experimental Research Center, Anhui University of Chinese Medicine, Hefei 230038, China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230013, China.
Molecules. 2022 Oct 7;27(19):6654. doi: 10.3390/molecules27196654.
It is generally believed that fresh (FDO) has more significant pharmacological activity than dried (DDO); however, the difference has not been clearly shown. Our study compared their antioxidant properties both in vitro and in vivo, and the molecular weight arrangement and monosaccharide composition of the fresh polysaccharides (FDOPs) and the dried polysaccharides (DDOPs) were analyzed by HPLC-GPC and GC-MS. The results showed that the FDO and its polysaccharides had more significant effects on scavenging DPPH, ABTS, and hydroxyl radicals than the DDO. In addition, both the FDO and DDO significantly reduced lipid peroxidation levels and increased the SOD, T-AOC, CAT, and GSH levels in mice with acute liver damage caused by CCl, while the FDO and its polysaccharides were more effective. Histopathological analysis further verified the protective effect of the Dendrobium polysaccharides on CCl-induced liver injury. The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO. Overall, our data identified differences in the antioxidant activities of the FDO and DDO, as well as the reasons for these differences.
一般认为新鲜铁皮石斛（FDO）比干铁皮石斛（DDO）具有更强的药理活性；然而，这种差异尚未得到明确证实。我们的研究比较了它们在体外和体内的抗氧化特性，并通过高效液相色谱-凝胶渗透色谱（HPLC-GPC）和气相色谱-质谱联用（GC-MS）分析了新鲜铁皮石斛多糖（FDOPs）和干铁皮石斛多糖（DDOPs）的分子量排列和单糖组成。结果表明，FDO 及其多糖对 DPPH、ABTS 和羟基自由基的清除作用比 DDO 更显著。此外，FDO 和 DDO 均能显著降低 CCl 所致急性肝损伤小鼠的脂质过氧化水平，提高 SOD、T-AOC、CAT 和 GSH 水平，而 FDO 及其多糖的效果更为显著。组织病理学分析进一步验证了铁皮石斛多糖对 CCl 诱导的肝损伤的保护作用。多糖含量测定表明，FDO 的多糖和甘露糖含量明显高于其干制品，FDO 中多糖的均匀排列在 DDO 中降解为三种不同分子量的多糖片段。总的来说，我们的数据确定了 FDO 和 DDO 的抗氧化活性存在差异，以及造成这种差异的原因。
