肺炎克雷伯菌 11 型中携带 KPC-2 及毒力因子的新型混合质粒的鉴定
Identification of a Novel Hybrid Plasmid Encoding KPC-2 and Virulence Factors in Klebsiella pneumoniae Sequence Type 11.
机构信息
Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China
出版信息
Antimicrob Agents Chemother. 2021 May 18;65(6). doi: 10.1128/AAC.02435-20.
Abstract
Recent emergence of carbapenem-resistant (CRKP) coharboring and pLVPK-like virulence plasmids represented a novel clinical challenge. In the present study, we characterized a and virulence hybrid plasmid, designated pCRHV-C2244, from a clinical ST11-K64 CRKP strain. pCRHV-C2244 was non-self-transmissible due to incomplete conjugative elements but mobilizable together with a conjugative helper. Enhanced virulence and stable maintenance without significant fitness loss in its original host were confirmed and .
摘要
最近出现的碳青霉烯类耐药（CRKP）同时携带 和 pLVPK 样毒力质粒代表了一种新的临床挑战。在本研究中，我们从临床 ST11-K64 CRKP 菌株中鉴定了一个 和毒力混合质粒，命名为 pCRHV-C2244。由于不完全的接合元件，pCRHV-C2244 是非自我传递的，但可以与一个接合辅助质粒一起移动。在其原始宿主中，增强的毒力和稳定的维持而没有明显的适应性损失得到了确认。
相似文献
1
Identification of a Novel Hybrid Plasmid Encoding KPC-2 and Virulence Factors in Klebsiella pneumoniae Sequence Type 11.
Antimicrob Agents Chemother. 2021 May 18;65(6). doi: 10.1128/AAC.02435-20.
2
Emergence of Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Coharboring a -Carrying Virulent Plasmid and a -Carrying Plasmid in an Egyptian Hospital.
mSphere. 2021 May 19;6(3):e00088-21. doi: 10.1128/mSphere.00088-21.
3
Emergence of IS26-mediated pLVPK-like virulence and NDM-1 conjugative fusion plasmid in hypervirulent carbapenem-resistant Klebsiella pneumoniae.
Infect Genet Evol. 2023 Sep;113:105471. doi: 10.1016/j.meegid.2023.105471. Epub 2023 Jun 21.
4
Characterization of a Multidrug-Resistant Porcine Klebsiella pneumoniae Sequence Type 11 Strain Coharboring and on Two Novel Hybrid Plasmids.
mSphere. 2019 Sep 11;4(5):e00590-19. doi: 10.1128/mSphere.00590-19.
5
Prevalence of Carbapenem-Resistant Co-Harboring blaKPC-Carrying Plasmid and pLVPK-Like Virulence Plasmid in Bloodstream Infections.
Front Cell Infect Microbiol. 2021 Mar 12;10:556654. doi: 10.3389/fcimb.2020.556654. eCollection 2020.
6
Microbial Characteristics and Genomic Analysis of an ST11 Carbapenem-Resistant Strain Carrying Conjugative Drug-Resistant Plasmid.
Front Public Health. 2022 Jan 27;9:809753. doi: 10.3389/fpubh.2021.809753. eCollection 2021.
7
Emergence of a novel conjugative hybrid virulence multidrug-resistant plasmid in extensively drug-resistant Klebsiella pneumoniae ST15.
Int J Antimicrob Agents. 2020 Jun;55(6):105952. doi: 10.1016/j.ijantimicag.2020.105952. Epub 2020 Apr 23.
8
Emergence of a Superplasmid Coharboring Hypervirulence and Multidrug Resistance Genes in Klebsiella pneumoniae Poses New Challenges to Public Health.
Microbiol Spectr. 2022 Dec 21;10(6):e0263422. doi: 10.1128/spectrum.02634-22. Epub 2022 Oct 20.
9
Mobilization of the nonconjugative virulence plasmid from hypervirulent Klebsiella pneumoniae.
Genome Med. 2021 Jul 22;13(1):119. doi: 10.1186/s13073-021-00936-5.
10
Nosocomial transmission and rearrangement of large resistance-virulence hybrid plasmids between two bacteremic ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae strains with low fitness cost.
Microb Pathog. 2022 Jul;168:105593. doi: 10.1016/j.micpath.2022.105593. Epub 2022 May 17.
引用本文的文献
1
Molecular and epidemiological characterization of carbapenem-resistant hypervirulent in Huaian, China (2022-2024): a retrospective study.
Front Cell Infect Microbiol. 2025 Jun 4;15:1569004. doi: 10.3389/fcimb.2025.1569004. eCollection 2025.
2
Mobile genetic elements encoding antibiotic resistance genes and virulence genes in : important pathways for the acquisition of virulence and resistance.
Front Microbiol. 2025 Feb 24;16:1529157. doi: 10.3389/fmicb.2025.1529157. eCollection 2025.
3
Hypervirulent (hv): Overview, Epidemiology, and Laboratory Detection.
Pathog Immun. 2025 Jan 24;10(1):80-119. doi: 10.20411/pai.v10i1.777. eCollection 2024.
4
Adaptive evolution of carbapenem-resistant hypervirulent in the urinary tract of a single patient.
Proc Natl Acad Sci U S A. 2024 Aug 27;121(35):e2400446121. doi: 10.1073/pnas.2400446121. Epub 2024 Aug 16.
5
Molecular characterization of hybrid virulence plasmids in ST11-KL64 KPC-2-producing multidrug-resistant hypervirulent from China.
Front Microbiol. 2024 Mar 14;15:1353849. doi: 10.3389/fmicb.2024.1353849. eCollection 2024.
6
RES-Xre toxin-antitoxin locus maintains the stability of the virulence plasmid in .
Emerg Microbes Infect. 2024 Dec;13(1):2316814. doi: 10.1080/22221751.2024.2316814. Epub 2024 Feb 23.
7
Drug Resistance and Molecular Characteristics of Carbapenem-Resistant OXA-48-Producing Strains in Hainan, China.
Microorganisms. 2023 Dec 27;12(1):49. doi: 10.3390/microorganisms12010049.
8
Clinical use of tigecycline may contribute to the widespread dissemination of carbapenem-resistant hypervirulent strains.
Emerg Microbes Infect. 2024 Dec;13(1):2306957. doi: 10.1080/22221751.2024.2306957. Epub 2024 Jan 30.
9
Genome sequencing unveils -harboring plasmids as drivers of enhanced resistance and virulence in nosocomial .
mSystems. 2024 Feb 20;9(2):e0092423. doi: 10.1128/msystems.00924-23. Epub 2024 Jan 9.
10
Emergence of OXA-48-producing hypervirulent Klebsiella pneumoniae strains in Taiwan.
Eur J Clin Microbiol Infect Dis. 2024 Feb;43(2):389-393. doi: 10.1007/s10096-023-04733-3. Epub 2023 Dec 7.
本文引用的文献
1
The Small Protein RmpD Drives Hypermucoviscosity in Klebsiella pneumoniae.
mBio. 2020 Sep 22;11(5):e01750-20. doi: 10.1128/mBio.01750-20.
2
Carbapenem Resistance-Encoding and Virulence-Encoding Conjugative Plasmids in Klebsiella pneumoniae.
Trends Microbiol. 2021 Jan;29(1):65-83. doi: 10.1016/j.tim.2020.04.012. Epub 2020 May 21.
3
Co-existence of a novel plasmid-mediated efflux pump with colistin resistance gene in one plasmid confers transferable multidrug resistance in .
Emerg Microbes Infect. 2020 Dec;9(1):1102-1113. doi: 10.1080/22221751.2020.1768805.
4
Population genomics of Klebsiella pneumoniae.
Nat Rev Microbiol. 2020 Jun;18(6):344-359. doi: 10.1038/s41579-019-0315-1. Epub 2020 Feb 13.
5
The Galleria mellonella Infection Model Does Not Accurately Differentiate between Hypervirulent and Classical Klebsiella pneumoniae.
mSphere. 2020 Jan 8;5(1):e00850-19. doi: 10.1128/mSphere.00850-19.
6
Evolution of hypervirulence in carbapenem-resistant Klebsiella pneumoniae in China: a multicentre, molecular epidemiological analysis.
J Antimicrob Chemother. 2020 Feb 1;75(2):327-336. doi: 10.1093/jac/dkz446.
7
Emergence of a hybrid plasmid derived from IncN1-F33:A-:B- and mcr-1-bearing plasmids mediated by IS26.
J Antimicrob Chemother. 2019 Nov 1;74(11):3184-3189. doi: 10.1093/jac/dkz327.
8
Hypervirulent Klebsiella pneumoniae.
Clin Microbiol Rev. 2019 May 15;32(3). doi: 10.1128/CMR.00001-19. Print 2019 Jun 19.
9
Convergence of virulence and MDR in a single plasmid vector in MDR Klebsiella pneumoniae ST15.
J Antimicrob Chemother. 2019 May 1;74(5):1218-1222. doi: 10.1093/jac/dkz028.
10
Phenotypic and Genotypic Characterization of Carbapenem-resistant Enterobacteriaceae: Data From a Longitudinal Large-scale CRE Study in China (2012-2016).
Clin Infect Dis. 2018 Nov 13;67(suppl_2):S196-S205. doi: 10.1093/cid/ciy660.
Zotero 插件