Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Endometriosis Research Center, Iran University of Medical Science, Tehran, Iran.
Sci Rep. 2021 Mar 15;11(1):6054. doi: 10.1038/s41598-021-85512-y.
Resveratrol is a phytochemical with anti-angiogenic, anti-inflammatory, and antioxidant properties. The present study has evaluated the effect of resveratrol on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β) and matrix metalloproteinase-9 (MMP-9) as factors related to endometriosis progression. Thirteen eutopic (EuESCs) and 8 ectopic (EESCs) endometrial stromal cells from women with endometriosis and 11 control endometrial stromal cells (CESCs) were treated with resveratrol (100 µM) for 6, 24 and 48 h. The gene and protein expression levels of VEGF, TGF-β, and MMP-9 were measured using real-time PCR and ELISA methods, respectively. Results showed that the basal gene and protein expression of VEGF and MMP-9 were higher in EESCs compared to EuESCs and CESCs (P < 0.01 to < 0.001 and P < 0.05 to < 0.01 respectively). Also, resveratrol treatment decreased the gene and protein expression of VEGF and MMP-9 in EuESCs, EESCs and CESCs (P < 0.05 to < 0.01 and P < 0.05 to < 0.01 respectively) and gene and protein expression of TGF-β in EESCs and EuESCs (P < 0.05 to < 0.01). The effect of resveratrol in reduction of VEGF gene expression was statistically more noticeable in EESCs compared to EuESCs and CESCs (P < 0.05). According to the findings, resveratrol may ameliorate endometriosis progression through reducing the expression of VEGF, TGF-β, and MMP-9 in endometrial stromal cells (ESCs).
白藜芦醇是一种具有抗血管生成、抗炎和抗氧化特性的植物化学物质。本研究评估了白藜芦醇对血管内皮生长因子（VEGF）、转化生长因子-β（TGF-β）和基质金属蛋白酶-9（MMP-9）表达的影响，这些因子与子宫内膜异位症的进展有关。从患有子宫内膜异位症的 13 名在位（EuESCs）和 8 名异位（EESCs）子宫内膜基质细胞和 11 名对照子宫内膜基质细胞（CESCs）中提取白藜芦醇（100 μM）处理 6、24 和 48 小时。使用实时 PCR 和 ELISA 方法分别测量 VEGF、TGF-β 和 MMP-9 的基因和蛋白表达水平。结果表明，EESCs 中 VEGF 和 MMP-9 的基础基因和蛋白表达均高于 EuESCs 和 CESCs（P < 0.01 至 < 0.001 和 P < 0.05 至 < 0.01）。此外，白藜芦醇处理降低了 EuESCs、EESCs 和 CESCs 中 VEGF 和 MMP-9 的基因和蛋白表达（P < 0.05 至 < 0.01 和 P < 0.05 至 < 0.01），以及 EESCs 和 EuESCs 中 TGF-β 的基因和蛋白表达（P < 0.05 至 < 0.01）。与 EuESCs 和 CESCs 相比，白藜芦醇在降低 EESCs 中 VEGF 基因表达方面的效果更为显著（P < 0.05）。根据研究结果，白藜芦醇可能通过降低子宫内膜基质细胞（ESCs）中 VEGF、TGF-β 和 MMP-9 的表达来改善子宫内膜异位症的进展。
