白藜芦醇处理对子宫内膜异位症与非子宫内膜异位症基质细胞中Bcl-2和Bax基因表达的影响。
The Effects of Resveratrol Treatment on Bcl-2 and Bax Gene Expression in Endometriotic Compared with Non-Endometriotic Stromal Cells.
作者信息
Kolahdouz-Mohammadi Roya, Delbandi Ali-Akbar, Khodaverdi Sepideh, Arefi Soheila, Arablou Tahereh, Shidfar Farzad
机构信息
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Public Health. 2020 Aug;49(8):1546-1554. doi: 10.18502/ijph.v49i8.3900.
BACKGROUND
We aimed to examine resveratrol effects on gene expression of Bcl-2, Bax and Bcl-2/Bax ratio in endometrial stromal cells derived from women with and without endometriosis.
METHODS
Endometrial tissues were obtained from 40 endometriotic patients and 15 non-endometriotic controls undergoing laparoscopic surgery or hysterectomy in the gynecology ward of Rassoul Akram Hospital, Tehran, Iran from 2015 to 2017. After the enzymatic digestion, eutopic (EuESCs) and ectopic (EESCs) endometrial stromal cells from patients with endometriosis as well as endometrial stromal cells from non-endometriotic controls (CESCs) were treated with or without resveratrol (100 μM) and the levels of Bcl-2, Bax and Bcl-2/Bax gene expression ratio in the cells from all origins were examined at 6, 24 and 48 h post-treatment by real-time PCR.
RESULTS
Resveratrol treatment increased Bcl-2 expression in CESCs at 24 and 48 h and in EuESCs at 48 h (<0.05), but had no significant effects on the expression of this gene in EESCs. On the other hand, resveratrol treatment increased Bax expression in EuESCs at 6 h and decreased its expression in EESCs at 48 h (<0.05). Regarding the Bcl-2/Bax gene expression ratio, resveratrol treatment increased Bcl-2/Bax gene expression ratio in CESCs and EuESCs at 48 h (<0.01). However, this treatment had no significant differential effect on Bcl-2 and Bcl-2/Bax gene expression ratio between CESCs and EuESCs at 48 h.
CONCLUSION
Resveratrol treatment significantly increased Bcl-2/Bax gene expression ratio in EuESCs and CESCs but had no significant effect in EESCs.
背景
我们旨在研究白藜芦醇对患有和未患有子宫内膜异位症女性的子宫内膜基质细胞中Bcl-2、Bax基因表达及Bcl-2/Bax比值的影响。
方法
2015年至2017年期间，从伊朗德黑兰拉苏勒·阿克兰医院妇科病房接受腹腔镜手术或子宫切除术的40例子宫内膜异位症患者和15例非子宫内膜异位症对照者获取子宫内膜组织。酶消化后，将子宫内膜异位症患者的在位（EuESCs）和异位（EESCs）子宫内膜基质细胞以及非子宫内膜异位症对照者的子宫内膜基质细胞（CESCs）用或不用白藜芦醇（100μM）处理，并在处理后6、24和48小时通过实时PCR检测所有来源细胞中Bcl-2、Bax基因表达水平及Bcl-2/Bax基因表达比值。
结果
白藜芦醇处理在24小时和48小时时增加了CESCs中Bcl-2的表达，在48小时时增加了EuESCs中Bcl-2的表达（<0.05），但对EESCs中该基因的表达无显著影响。另一方面，白藜芦醇处理在6小时时增加了EuESCs中Bax的表达，在48小时时降低了EESCs中Bax的表达（<0.05）。关于Bcl-2/Bax基因表达比值，白藜芦醇处理在48小时时增加了CESCs和EuESCs中Bcl-2/Bax基因表达比值（<0.01）。然而，该处理在48小时时对CESCs和EuESCs之间的Bcl-2及Bcl-2/Bax基因表达比值无显著差异影响。
结论
白藜芦醇处理显著增加了EuESCs和CESCs中Bcl-2/Bax基因表达比值，但对EESCs无显著影响。
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