Department of Neurosurgery, Chongqing General Hospital, University of Chinese Academy of Science, 118 Xingguang Avenue, Liangjiang New Area, Chongqing, 400016, China.
J Mol Histol. 2021 Jun;52(3):589-596. doi: 10.1007/s10735-021-09966-0. Epub 2021 Mar 16.
Glioblastoma (GBM) is a deadly brain tumor with a bleak prognosis. In recent years, the copine III (CPNE3) protein was discovered to be associated to metastasis across various types of malignancies. Nevertheless, its function has not been well documented in glioma. This study characterizes CPNE3 expression in GBM along with its impact and underlying molecular mechanism with regards to cellular migration, invasion and proliferation. Immunohistochemistry was used to characterizes CPNE3 expression in the glioma tissues. Then, knockdown of CPNE3 expression was used to analyze the role of CPNE3 in GBM cell viability, migration, invasion. Western blot analysis was performed to measure the protein levels of FAK signaling pathway. We found that GBM tissues had higher CPNE3 expressions as compared to those in normal brain tissues. CPNE3 silencing in GBM cells impaired the migratory, invasive and proliferative abilities of GBM cells that can be attributed to inactivation of the FAK signaling pathway. Collectively, these findings highlight the role of CPNE3 as a new biomarker, offering deeper insights into its carcinogenic role in GBM.
胶质母细胞瘤（GBM）是一种预后不良的致命脑肿瘤。近年来，发现连蛋白 III（CPNE3）蛋白与各种类型恶性肿瘤的转移有关。然而，其在神经胶质瘤中的功能尚未得到很好的阐述。本研究描述了 CPNE3 在 GBM 中的表达及其对细胞迁移、侵袭和增殖的影响及潜在的分子机制。免疫组织化学用于描述神经胶质瘤组织中 CPNE3 的表达。然后，敲低 CPNE3 的表达，分析 CPNE3 在 GBM 细胞活力、迁移、侵袭中的作用。Western blot 分析用于测量 FAK 信号通路的蛋白水平。我们发现，与正常脑组织相比，GBM 组织中 CPNE3 的表达更高。CPNE3 在 GBM 细胞中的沉默削弱了 GBM 细胞的迁移、侵袭和增殖能力，这可归因于 FAK 信号通路的失活。总之，这些发现强调了 CPNE3 作为一个新的生物标志物的作用，为其在 GBM 中的致癌作用提供了更深入的见解。
