Faculty of Veterinary Medicine, Institute of Preclinical Veterinary Sciences, Sub-Department of Veterinary Microbiology, University of Life Sciences, Lublin, Poland.
J Appl Microbiol. 2021 Oct;131(4):2061-2071. doi: 10.1111/jam.15074. Epub 2021 Mar 25.
The aim of the study was to assess resistance and virulence of Enterococcus faecalis isolated from the gastrointestinal tract of dogs and cats, analyse their genotypic variability and estimate the correlation between the occurrence of antimicrobial resistance, virulence determinants and genotypic profiles.
The susceptibility of E. faecalis to penicillin, ampicillin, vancomycin, erythromycin, tetracycline, ciprofloxacin, gentamicin, streptomycin and kanamycin was determined by the broth microdilution method. The isolates were tested for the presence of selected genes encoding resistance to macrolides, tetracyclines, aminoglycosides and glycopeptides as well as genes encoding virulence factors. Genotyping was performed using the ADSRRS-fingerprinting method. The highest percentage of resistant strains was observed in relation to erythromycin (96%), ciprofloxacin (93%) and tetracycline (82%). High percentage of strains resistant to high-level aminoglycosides was noted (kanamycin-33%, gentamicin-29%, streptomycin-24%), as well as multidrug-resistant (78%). The genotypic analysis of E. faecalis showed high heterogeneity of genotypic profiles (37) correlating with some resistance profiles. The most common virulence genes amongst E. faecalis were efaAfs (93%), cpd, ccf and cob (86%).
The results of this study confirm that companion animals should be considered as a reservoir of E. faecalis carrying resistance and virulence determinants.
本研究旨在评估从犬猫胃肠道分离的屎肠球菌的耐药性和毒力，分析其基因型变异性，并评估抗菌药物耐药性、毒力决定因子和基因型之间的相关性。
采用肉汤微量稀释法测定屎肠球菌对青霉素、氨苄西林、万古霉素、红霉素、四环素、环丙沙星、庆大霉素、链霉素和卡那霉素的敏感性。检测了所选编码耐大环内酯类、四环素类、氨基糖苷类和糖肽类抗生素以及编码毒力因子的基因的存在情况。采用 ADSRRS 指纹图谱法进行基因分型。对红霉素（96%）、环丙沙星（93%）和四环素（82%）耐药的菌株比例最高。高水平氨基糖苷类耐药（卡那霉素-33%、庆大霉素-29%、链霉素-24%）和多药耐药（78%）的菌株比例较高。屎肠球菌的基因分析显示基因型图谱具有高度的异质性（37 种），与某些耐药图谱相关。屎肠球菌中最常见的毒力基因是 efaAfs（93%）、cpd、ccf 和 cob（86%）。
本研究结果证实，伴侣动物应被视为携带耐药性和毒力决定因子的屎肠球菌的储主。
