Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
THQ Hospital Karor Lal Esan Layyah, Karor, Karor Lal Esan, Layyah, Pakistan.
Biomed Res Int. 2022 Sep 27;2022:8101866. doi: 10.1155/2022/8101866. eCollection 2022.
This study was designed to identify from clinical mastitis of cattle and determine their antimicrobial resistance and virulence determinants to evaluate their potential public health significance. A total of 105 composite milk samples (80 from cattle with clinical mastitis and 25 from apparently healthy cattle) were analyzed. were isolated by culturing on enterococcal selective media and identified by PCR and sequencing. Antimicrobial resistance phenotype was elucidated by the disc diffusion method, and MIC was determined by broth microdilution method according to CLSI guidelines. Detection of antimicrobial resistance and virulence genes was done by PCR. were isolated from 11.25% (9/80) of the clinical mastitis and 4% (1/25) of the apparently healthy cattle milk samples. The disc diffusion test revealed 40% isolates as resistant to tetracycline and azithromycin, respectively. Among them, 20% (2/10) of isolates showed resistance to both tetracycline and azithromycin. Tetracycline-resistant isolates showed MIC ranging from ≥64 to >128 g/ml and carried tetracycline-resistant genes , , and in 25%, 25%, and 50% of the resistant isolates, respectively. On the other hand, all the isolates were sensitive to amoxicillin, ampicillin, bacitracin, chloramphenicol, gentamicin, penicillin, and vancomycin. In addition, the isolates carried at least one of the nine virulence genes screened with having the highest frequency, followed by , , , , , and genes. Positive correlations were evident between , , , and genes that are associated with the attachment and biofilm formation in isolated in this study carried antibiotic resistance and virulence determinants which explain their competence to be potential human pathogens.
本研究旨在从牛临床乳腺炎中分离鉴定 ，并确定其抗菌药物耐药性和毒力决定因素，以评估其潜在的公共卫生意义。共分析了 105 份复合牛奶样本（80 份来自患有临床乳腺炎的奶牛，25 份来自明显健康的奶牛）。通过在肠球菌选择性培养基上培养分离 ，通过 PCR 和测序进行鉴定。采用纸片扩散法阐明抗菌药物耐药表型，根据 CLSI 指南通过肉汤微量稀释法确定 MIC。采用 PCR 检测抗菌药物耐药和毒力基因。从 11.25%（9/80）的临床乳腺炎和 4%（1/25）的明显健康奶牛乳样中分离到 。纸片扩散试验显示 40%的分离株分别对四环素和阿奇霉素耐药。其中，20%（2/10）的分离株对四环素和阿奇霉素均耐药。四环素耐药分离株的 MIC 范围为≥64 至>128 μg/ml，携带四环素耐药基因 、 、 和 ，分别在 25%、25%和 50%的耐药分离株中。另一方面，所有分离株均对阿莫西林、氨苄西林、杆菌肽、氯霉素、庆大霉素、青霉素和万古霉素敏感。此外，在所筛选的 9 个毒力基因中，所有分离株均携带至少一个，其中 携带频率最高，其次是 、 、 、 、 和 基因。在本研究中分离到的与附着和生物膜形成相关的 、 、 和 基因呈正相关，这些基因携带抗菌药物耐药性和毒力决定因素，这解释了它们作为潜在人类病原体的能力。
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