Functional Foods and Nutraceuticals Research Laboratory, Biochemistry Department, Federal University of Technology, Akure, Nigeria.
Science Laboratory Technology Department (Biochemistry Unit), Ede, Osun State, Nigeria.
J Basic Clin Physiol Pharmacol. 2021 Mar 16;33(4):389-397. doi: 10.1515/jbcpp-2020-0173.
Several studies had been conducted to examine the link between diabetes and diabetes encephalopathy. This study was conducted to examine the potency of berberine (BER) on the restoration of impaired neurochemicals in the brain of streptozotocin (STZ)-induced diabetic Wistar rats.
Fifty-six (56) adult rats weighing between 200 and 230 g were randomly divided into seven groups (n=8) as follows; Group I is normal control; Groups II and III were normal rats treated with 50 and 100 mg/kg respectively; Group IV-VII were STZ-induced rats, but Groups V-VII were treated with acarbose (25 mg/kg), 50 and 100 mg/kg of BER, respectively.
The result of the study showed that untreated STZ-induced diabetic rats have increased acetylcholinesterase (AChE), butyrylcholinesterase (BChE), monoamine oxidase (MAO) activities, and malonylaldehyde (MDA) level, with concomitant decrease of superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) level. However, daily treatment with 50 and 100 mg/kg BER and ACA significantly reversed these effects.
The findings of this study clearly indicated that BER possesses neuro-protective and antioxidative potentials and normalize neurochemical impairment distort by diabetes.
已有多项研究探讨了糖尿病与糖尿病性脑病之间的关系。本研究旨在检验小檗碱（BER）对链脲佐菌素（STZ）诱导的糖尿病 Wistar 大鼠脑内受损神经化学物质恢复的功效。
56 只成年大鼠（体重在 200 到 230 克之间）被随机分为 7 组（每组 8 只）：I 组为正常对照组；II 组和 III 组分别给予 50 毫克/千克和 100 毫克/千克的 BER 作为正常对照处理；IV 组-VII 组为 STZ 诱导的糖尿病大鼠，但 V 组-VII 组分别用阿卡波糖（25 毫克/千克）、50 毫克/千克和 100 毫克/千克的 BER 进行治疗。
研究结果表明，未经治疗的 STZ 诱导的糖尿病大鼠乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、单胺氧化酶（MAO）活性增加，丙二醛（MDA）水平升高，而过氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）活性和谷胱甘肽（GSH）水平降低。然而，每日给予 50 毫克/千克和 100 毫克/千克的 BER 和 ACA 可显著逆转这些作用。
本研究结果清楚地表明，BER 具有神经保护和抗氧化潜力，可纠正糖尿病引起的神经化学物质损伤。
