Department of Biochemistry, Federal University of Technology, Akure, Ondo-State, Nigeria.
Department of Biomedical Technology, Federal University of Technology, Akure, Ondo-State, Nigeria.
Metab Brain Dis. 2018 Apr;33(2):457-466. doi: 10.1007/s11011-018-0198-2. Epub 2018 Feb 12.
There are strong correlations between diabetes mellitus and cognitive dysfunction. This study sought to investigate the modulatory effects of Moringa oleifera leaf (ML) and seed (MS) inclusive diets on biomolecules [acetylcholinesterase (AChE), butyrylcholinesterase (BChE)] angiotensin-I converting enzyme (ACE), arginase, catalase, glutathione transferase (GST) and glutathione peroxidase (GSH-Px) activities, glutathione (GSH) and nitric oxide (NO) levels] associated with cognitive function in the brain of streptozotocin (STZ)-induced diabetic rats treated with acarbose (ACA). The rats were made diabetic by intraperitoneal administration of 0.1 M sodium-citrate buffer (pH 4.5) containing STZ [60 mg/kg b.w (BW)] and fed with diets containing 2 and 4% ML/MS. Acarbose (25 mg/kg BW) was administered by gavage daily for 14 days. The animals were distributed in eleven groups of eight animals as follows: control, STZ-induced, STZ + ACA, STZ + 2% ML, STZ + ACA + 2% ML, STZ + 4% ML, STZ + ACA + 4% ML, STZ + 2% MS, STZ + ACA + 2% MS, STZ + 4% MS, STZ + ACA + 4% MS. There were marked increase in AChE, BChE, arginase, ACE and concomitant decrease in catalase, GST, GSH-Px, activities and NO levels in STZ-diabetic group compared with the control. However, there was a decrease in AChE, BChE and ACE activities and concomitant increase in the antioxidant molecules in the groups fed with supplemented diets treated with/without ACA compared with the STZ-diabetic group. These findings suggest that ML/MS supplemented diet could prevent cognitive dysfunction-induced by chronic hyperglycemia.
糖尿病与认知功能障碍之间存在很强的相关性。本研究旨在探讨辣木叶（ML）和种（MS）综合饮食对生物分子[乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）]、血管紧张素转换酶（ACE）、精氨酸酶、过氧化氢酶、谷胱甘肽转移酶（GST）和谷胱甘肽过氧化物酶（GSH-Px）活性、谷胱甘肽（GSH）和一氧化氮（NO）水平的调节作用，这些生物分子与链脲佐菌素（STZ）诱导的糖尿病大鼠大脑中的认知功能有关，并接受阿卡波糖（ACA）的治疗。通过腹腔内给予含 STZ [60mg/kg 体重（BW）]的 0.1 M 柠檬酸钠缓冲液（pH 4.5）使大鼠产生糖尿病，并给予含有 2%和 4% ML/MS 的饮食。阿卡波糖（25mg/kg BW）每天通过灌胃给药 14 天。动物分为十一组，每组 8 只，如下所示：对照组、STZ 诱导组、STZ+ACA 组、STZ+2% ML 组、STZ+ACA+2% ML 组、STZ+4% ML 组、STZ+ACA+4% ML 组、STZ+2% MS 组、STZ+ACA+2% MS 组、STZ+4% MS 组、STZ+ACA+4% MS 组。与对照组相比，STZ 糖尿病组的 AChE、BChE、精氨酸酶、ACE 显著增加，而过氧化氢酶、GST、GSH-Px 活性和 NO 水平则相应降低。然而，与 STZ 糖尿病组相比，给予补充饮食的各组（用/不用 ACA 处理）的 AChE、BChE 和 ACE 活性降低，而抗氧化分子增加。这些发现表明，ML/MS 补充饮食可预防慢性高血糖引起的认知功能障碍。
