Feng Y, Halaris A E, Piletz J E
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson 39216, USA.
J Chromatogr B Biomed Sci Appl. 1997 Apr 11;691(2):277-86. doi: 10.1016/s0378-4347(96)00458-6.
Decarboxylated arginine, agmatine, is a neurotransmitter candidate for imidazoline receptors. A method is described to measure agmatine in rat brain and human plasma by isocratic high-performance liquid chromatography (HPLC) with fluorescence detection and o-phthalaldehyde derivatization. Quantitation is based on the method of additions of internal agmatine spikes. This assay has sensitivity in the low picomole range and a detection limit of 100 fmol. The correlation coefficient for the agmatine standard curve was 0.999+/-0.001 S.D., and intra- and inter-assay C.V.s were less than 8%. The accuracy of our isocratic method compared favorably with a gradient HPLC protocol, originally developed for bacterial agmatine, which we modified for use with tissues. Agmatine concentrations in rat brain were proportioned similarly to the regional distribution of imidazoline-1 receptors. These methods can be used as reliable research tools in various biological samples.
脱羧精氨酸，即胍丁胺，是一种咪唑啉受体的神经递质候选物。本文描述了一种通过等度高效液相色谱（HPLC）结合荧光检测和邻苯二甲醛衍生化来测定大鼠脑和人血浆中胍丁胺的方法。定量基于加入内标胍丁胺峰的方法。该测定法在低皮摩尔范围内具有灵敏度，检测限为100飞摩尔。胍丁胺标准曲线的相关系数为0.999±0.001标准差，批内和批间变异系数小于8%。我们的等度方法的准确性与最初为细菌胍丁胺开发的梯度HPLC方案相比具有优势，我们对该方案进行了修改以用于组织。大鼠脑中胍丁胺的浓度与咪唑啉-1受体的区域分布相似。这些方法可作为各种生物样品中可靠的研究工具。
