Istanbul Technical University, Department of Chemical Engineering, Istanbul, Turkey.
Istanbul Technical University, Polymer Science and Technology, Istanbul, Turkey.
Eur J Pharm Biopharm. 2021 May;162:59-69. doi: 10.1016/j.ejpb.2021.02.009. Epub 2021 Mar 13.
Development of drug delivery systems is an extensively researched area in biomedical field. In recent years, there is an increasing interest on fabrication of biocompatible nanofibrous drug delivery systems. In the present study, poly(ω-pentadecalactone-co-ε-caprolactone)/gelatin nanofibrous membranes were fabricated for the controlled delivery and release of tetracycline hydrochloride (TCH) antibiotic. Poly(ω-pentadecalactone-co-ε-caprolactone) content provides an originality to the membrane, since it has been synthesized enzymatically previously. Varied amounts of tetracycline hydrochloride including poly(ω-pentadecalactone-co-ε-caprolactone)/gelatin (1:1, v:v) binary polymer blend was electrospun and characterizations (morphological and molecular structure, wettability characteristics, and thermal behavior) were applied to investigate the incorporation of drug molecule. Afterwards, in vitro drug release studies were carried out and mathematical modelling was applied to drug release data in order to clarify the transport mechanism of drug. TCH release profile comprised of an initial burst release in first hour and followed by a sustained release through 14 days which allowed sufficient antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus and Bacillus subtilis) bacteria. The presented drug delivery system may be applied as an antibacterial wound dressing device for skin infections.
药物输送系统的开发是生物医学领域中一个广泛研究的领域。近年来，人们对制造生物相容性的纳米纤维药物输送系统越来越感兴趣。在本研究中，制备了聚（ω-十五内酯-共-ε-己内酯）/明胶纳米纤维膜，用于盐酸四环素（TCH）抗生素的控制释放。聚（ω-十五内酯-共-ε-己内酯）的含量为膜提供了新颖性，因为它之前是通过酶合成的。包括聚（ω-十五内酯-共-ε-己内酯）/明胶（1:1，v:v）二元聚合物共混物在内的不同含量的盐酸四环素通过静电纺丝进行了纺丝，并进行了表征（形态和分子结构、润湿性特性和热行为）以研究药物分子的掺入。随后，进行了体外药物释放研究，并对药物释放数据进行了数学建模，以阐明药物的传输机制。TCH 释放曲线包括最初的 1 小时内的突释，随后通过 14 天的持续释放，这使得该药物对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌和枯草芽孢杆菌）细菌都具有足够的抗菌活性。所提出的药物输送系统可作为用于皮肤感染的抗菌伤口敷料装置。
