Han Huiming, Zhao Zhi, Lin Yan, Lin Baihui, Xu Hao, Zheng Beiwen
School of Basic Medical Sciences, Beihua University, Jilin, Jilin Province, People's Republic of China.
Department of Neonatology, Shaanxi Province People's Hospital, Xi'an, Shaanxi Province, People's Republic of China.
Infect Drug Resist. 2022 Mar 19;15:1127-1133. doi: 10.2147/IDR.S347943. eCollection 2022.
In this study, we describe, for the first time, the co-existence of and in a carbapenem-resistant strain DY2019 isolated from a patient with urinary tract infection in China. We aimed to investigate the genomic context of two β-lactamase-producing plasmids and characterize the transmission mechanism of the carbapenemase-encoding gene. Whole-genome sequencing of strain DY2019 was performed with Nanopore and Illumina platforms, which revealed a chromosome sequence with the length of 4,830,928 bp, an IncC group plasmid pDY2019-OXA (size of 178,134 bp), and a novel IncHI2 group plasmid pDY2019-NDM (length 348,495 bp). A total of 16 antimicrobial resistance genes (ARGs) that confer resistance to nine different antibiotic groups were identified in strain DY2019, and 11 of them were carried by plasmid pDY2019-OXA. These data and analyses suggest that the carbapenem-resistant strains may serve as potential reservoir of carbapenemase and highlight the need for further close surveillance of this species in clinical settings.
在本研究中，我们首次描述了在中国从一名尿路感染患者中分离出的耐碳青霉烯类肺炎克雷伯菌菌株DY2019中[此处原文有缺失内容]的共存情况。我们旨在研究两个产β-内酰胺酶质粒的基因组背景，并表征碳青霉烯酶编码基因的传播机制。使用纳米孔和Illumina平台对菌株DY2019进行了全基因组测序，结果显示其染色体序列长度为4,830,928 bp，一个IncC组质粒pDY2019 - OXA（大小为178,134 bp），以及一个新型IncHI2组质粒pDY2019 - NDM（长度为348,495 bp）。在菌株DY2019中总共鉴定出16个赋予对九种不同抗生素组耐药性的抗菌抗性基因（ARGs），其中11个由质粒pDY2019 - OXA携带。这些数据和分析表明，耐碳青霉烯类肺炎克雷伯菌菌株可能是碳青霉烯酶的潜在储存库，并突出了在临床环境中对该菌种进行进一步密切监测的必要性。
