Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Mol Biol Rep. 2021 Mar;48(3):2325-2333. doi: 10.1007/s11033-021-06262-8. Epub 2021 Mar 16.
Pseudomonas aeruginosa is a ubiquitous bacterium, which is able to change its physiological characteristics in response to different habitats. Environmental strains are presumably less pathogenic than clinical strains and whether or not the clinical strains originate from the environment or through inter-host transmission remains poorly understood. To minimize the risk of infection, a better understanding of proteomic profiling of P. aeruginosa is necessary for elucidating the correlation between environmental and clinical strains. Based on antimicrobial susceptibility and patterns of virulence, we selected 12 clinical and environmental strains: (i) environmental, (ii) multidrug resistant (MDR) clinical and (iii) susceptible clinical strains. Whole-cell protein was extracted from each strain and subjected to two-dimensional differential gel electrophoresis (2-D DIGE) and liquid chromatography tandem mass spectrometry quadrupole time-of-flight (LC-MS QTOF). All 12 strains were clustered into 3 distinct groups based on their variance in protein expression. A total of 526 matched spots were detected and four differentially expressed protein spots (p < 0.05) were identified and all differential spots were downregulated in MDR strain J3. Upregulation of chitin binding and BON domain proteins was present in the environmental and some MDR strains, whereas the clinical strains exhibited distinct proteomic profiles with increased expression of serine protein kinase and arginine/ornithine transport ATP-binding proteins. Significant difference in expression was observed between susceptible clinical and MDR strains, as well as susceptible clinical and environmental strains. Transition from an environmental saprophyte to a clinical strain could alter its physiological characteristics to further increase its adaptation.
铜绿假单胞菌是一种无处不在的细菌，能够根据不同的栖息地改变其生理特征。环境株的致病性可能比临床株低，而临床株是来自环境还是通过宿主间传播尚不清楚。为了最大限度地降低感染风险，需要更好地了解铜绿假单胞菌的蛋白质组学特征，以阐明环境株和临床株之间的相关性。基于抗菌药物敏感性和毒力模式，我们选择了 12 株临床和环境分离株：（i）环境株，（ii）多药耐药（MDR）临床株和（iii）敏感临床株。从每株菌中提取全细胞蛋白，进行二维差异凝胶电泳（2-D DIGE）和液相色谱串联质谱四极杆飞行时间（LC-MS QTOF）分析。根据蛋白质表达的差异，将所有 12 株菌聚类为 3 个不同的组。共检测到 526 个匹配斑点，鉴定出 4 个差异表达蛋白斑点（p < 0.05），并且 MDR 菌株 J3 中的所有差异斑点均下调。在环境株和一些 MDR 株中存在几丁质结合和 BON 结构域蛋白的上调，而临床株则表现出不同的蛋白质组学特征，丝氨酸蛋白激酶和精氨酸/鸟氨酸转运 ATP 结合蛋白的表达增加。敏感临床株和 MDR 株之间以及敏感临床株和环境株之间观察到表达的显著差异。从环境腐生菌向临床菌株的转变可能会改变其生理特征，从而进一步增强其适应性。
