School of Sport Science, Beijing Sport University, No.48 Xinxi Road, Beijing, PR China.
Food & Drug Doping Control Laboratory, China Anti-Doping Agency, No.1 Anding Road, Beijing, PR China.
Bioanalysis. 2021 Apr;13(7):587-604. doi: 10.4155/bio-2021-0019. Epub 2021 Mar 17.
A series of dried blood spot (DBS) detection methods for doping agents have been developed in the last two decades. The DBS technique minimizes invasiveness and reduces storage and shipping costs. Recently, the World Anti-Doping Agency announced the use of DBS for the 2022 Beijing Winter Olympic Games and Paralympic Games owing to the advantages of the DBS application in routine doping control. Therefore the further development of detection methods for doping agents in DBS is important and urgent. This review summarizes five aspects of DBS application in doping analysis: sample collection, storage conditions, pretreatment, instrumentation and validation according to the Prohibited List issued by the World Anti-Doping Agency, and proposes some suggestions for future studies of DBS in doping analysis.
过去二十年来，人们开发了一系列用于检测兴奋剂的干血斑（DBS）检测方法。DBS 技术最大限度地减少了创伤，降低了储存和运输成本。最近，世界反兴奋剂机构（WADA）宣布在 2022 年北京冬奥会和冬残奥会期间使用 DBS，这是因为 DBS 在常规兴奋剂检测中的应用具有优势。因此，DBS 中兴奋剂检测方法的进一步发展是重要且紧迫的。本综述根据世界反兴奋剂机构发布的禁用清单，从 DBS 在兴奋剂分析中的应用方面（包括样本采集、储存条件、预处理、仪器和验证）进行了总结，并对未来 DBS 在兴奋剂分析中的研究提出了一些建议。
