Microbiology and Functionality Research Group and Industrial Technology Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.
Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 02841, Republic of Korea.
Antonie Van Leeuwenhoek. 2021 May;114(5):507-513. doi: 10.1007/s10482-021-01536-4. Epub 2021 Mar 17.
Strain CBA3638 was isolated from the Geum River sediment, Republic of Korea. The cells of strain CBA3638 were Gram-stain-positive, strictly anaerobic, rod-shaped, and 0.5-1.0 μm wide, and 4.0-4.5 μm long. Optimal growth occurred at 37 °C, pH 7.0, and 1.0% (w/v) NaCl. Based on the 16S rRNA gene sequence, the phylogenetic analysis showed that strain CBA3638 belongs to the genus Anaerocolumna in the family Lachnospiraceae, and is most closely related to Anaerocolumna cellulosilytica (94.6-95.0%). The DDH value with A. cellulosilytica SN021 showed 15.0% relatedness. The genome of strain CBA3638 consisted of one circular chromosome that is 5,500,435 bp long with a 36.7 mol% G + C content. The genome contained seven 16S-5S-23S rRNA operons and one antibiotic resistance-related transporter gene (mefA). Quinones were not detected. The predominant cellular fatty acids were C and C and the polar lipids were diphosphatidylglycerol, phosphatidylcholine, and uncharacterised polar lipids. Based on the polyphasic taxonomic analysis, we propose strain CBA3638 as a novel species in the genus Anaerocolumna, with the name Anaerocolumna sedimenticola sp. nov. The type strain is CBA3638 (= KACC 21652 = DSM 110663).
CBA3638 菌株从韩国 Geum 河沉积物中分离得到。该菌株的细胞呈革兰氏阳性，严格厌氧，杆状，宽 0.5-1.0μm，长 4.0-4.5μm。最佳生长条件为 37°C、pH7.0 和 1.0%（w/v）NaCl。根据 16S rRNA 基因序列，系统发育分析表明，CBA3638 菌株属于拉恩氏菌科 Anaerocolumna 属，与 Anaerocolumna cellulosilytica（94.6-95.0%）最为密切相关。与 A. cellulosilytica SN021 的 DDH 值显示为 15.0%的相关性。CBA3638 菌株的基因组由一条大小为 5,500,435bp 的环状染色体组成，GC 含量为 36.7mol%。基因组包含七个 16S-5S-23S rRNA 操纵子和一个抗生素抗性相关转运基因（mefA）。未检测到醌类物质。主要的细胞脂肪酸为 C 和 C，极性脂类为双磷脂酰甘油、磷脂酰胆碱和未鉴定的极性脂类。基于多相分类学分析，我们提出 CBA3638 菌株为 Anaerocolumna 属的一个新种，命名为 Anaerocolumna sedimenticola sp. nov. 。模式菌株为 CBA3638（=KACC 21652=DSM 110663）。
