Department of Dermatology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou Guangdong, 510515, China.
Drug Deliv Transl Res. 2022 Jan;12(1):325-332. doi: 10.1007/s13346-021-00942-5. Epub 2021 Mar 17.
Biofilm formation by Propionibacterium acnes is known to cause failure of anti-acne treatment. Conventional therapies for acne are typically inadequate. Accordingly, in this study, we evaluated the therapeutic potential of photodynamic therapy (PDT) using hexyl-aminolevulinate (HAL)-loaded ethosomes (ESs) against the biofilms of P. acnes in vitro and P. acnes-induced inflammatory acne model in vivo. The antibacterial effects of HAL ESs were evaluated using XTT colorimetric assays and scanning electron microscopic observations of morphological changes. P. acnes was intradermally injected into the ears of Sprague-Dawley rats, and the anti-inflammatory effects of HAL ESs were measured by determining changes in appearance, histology, and the antibacterial effects by P. acnes abundance in ear tissues compared with blank control ESs, HAL alone, and 5-aminolevulinic acid (ALA) alone. The highest reduction in viability in P. acnes biofilms was observed after treatment with 5 mg/mL HAL ESs. Notably, blank control ESs also showed significant inhibitory effects. Furthermore, HAL ESs had superior therapeutic effects in the rat model compared with HAL or ALA solutions. The observed therapeutic effects of HAL ESs against P. acnes biofilms and P. acnes-induced inflammation suggest that PDT with HAL-loaded ESs may have potential applications in the treatment of acne.
痤疮丙酸杆菌的生物膜形成已知会导致抗痤疮治疗失败。痤疮的常规治疗通常效果不足。因此，在这项研究中，我们评估了载六氢氨基乙酸（HAL）的乙基己基琥珀酸酯（ES）光动力疗法（PDT）对体外痤疮丙酸杆菌生物膜和体内痤疮丙酸杆菌诱导的炎症性痤疮模型的治疗潜力。通过 XTT 比色法和形态变化的扫描电子显微镜观察评估了 HAL ES 的抗菌效果。将痤疮丙酸杆菌皮内注射到 Sprague-Dawley 大鼠的耳朵中，通过比较空白对照 ES、HAL 单独和 5-氨基乙酰丙酸（ALA）单独与耳组织中 P. acnes 丰度来测量外观、组织学和抗菌效果的变化，从而评估 HAL ES 的抗炎作用。在用 5 mg/mL HAL ES 处理后，观察到对 P. acnes 生物膜的活力降低最明显。值得注意的是，空白对照 ES 也表现出显著的抑制作用。此外，与 HAL 或 ALA 溶液相比，HAL ES 在大鼠模型中具有更好的治疗效果。HAL ES 对 P. acnes 生物膜和 P. acnes 诱导的炎症的治疗效果表明，载 HAL 的 ESs 的 PDT 可能在痤疮治疗中有应用潜力。
