Thilakarathna G C, Navaratne S B, Wickramasinghe I, Ranasinghe P, Samarkoon S R, Samarasekera J K R R
Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka; Department of Animal and Food Sciences, Faculty of Agriculture, Rajarata University of Sri Lanka, Anuradhapura, Sri Lanka.
Department of Food Science and Technology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
J Ayurveda Integr Med. 2021 Apr-Jun;12(2):261-268. doi: 10.1016/j.jaim.2020.10.010. Epub 2021 Mar 14.
The excessive formation of Advanced Glycation End-products (AGEs) by non-enzymatic glycation mediates many health complications in the human body and the formation of AGEs largely accelerated under the hyperglycaemic condition.
The prospect of the study to assess the strength of inhibiting the rapid AGE formations in four Ayurvedic medicinal plants, namely; Salacia reticulata (stems), Syzygium cumini (barks), Artocarpus heterophyllus (mature leaves) and, Cassia auriculata (flowers).
Herbal decoctions of four medicinal plant materials were prepared by simmering with hot water as prescribed by the Ayurvedic medicine. The effectiveness of the decoctions was analyzed in vitro based on their Anti-AGE formation activity, glycation reversing, and anti-oxidant potentials.
According to the results, the decoctions of S. reticulata, A. heterophyllus and C. auriculata indicated the strong Anti-AGE forming (IC: 23.01 ± 2.70, 32.01 ± 2.09, 43.66 ± 2.11 mg/mL, respectively), glycation reversing (EC: 183.15 ± 7.67, 91.85 ± 1.93, 252.35 ± 4.03 mg/mL, respectively) and antioxidant potentials in terms of total polyphenol content (TPC), total flavonoid content (TFC), ferric ion reducing power (FRAP), ABTS and DPPH radical scavenging activities. However, the decoction of S. cumini reported the significantly high (p < 0.05) Anti-AGE forming, (IC: 9.75 ± 0.32 mg/mL), glycation reversing (EC: 66.45 ± 4.51 mg/mL), and antioxidant potentials against the decoctions of the other three plant materials.
S. cumini bark extract was identified as the best source in controlling the formation of AGEs excessively. Further, the other three plant extracts can also be effectively used as potential therapeutic agents to control the pathological conditions associated with AGEs-mediated health complications.
非酶糖基化作用导致晚期糖基化终产物（AGEs）过度形成，介导了人体许多健康并发症，且在高血糖条件下AGEs的形成会大大加速。
本研究旨在评估四种阿育吠陀药用植物，即网状五层龙（茎）、乌墨（树皮）、木菠萝（成熟叶）和耳叶决明（花）抑制AGEs快速形成的能力。
按照阿育吠陀医学的规定，用热水熬煮四种药用植物材料制备草药汤剂。基于其抗AGEs形成活性、糖化逆转能力和抗氧化潜力，对汤剂的有效性进行体外分析。
结果显示，网状五层龙、木菠萝和耳叶决明的汤剂表现出较强的抗AGEs形成能力（IC50分别为23.01±2.70、32.01±2.09、43.66±2.11mg/mL）、糖化逆转能力（EC50分别为183.15±7.67、91.85±1.93、252.35±4.03mg/mL）以及抗氧化潜力，具体表现为总多酚含量（TPC）、总黄酮含量（TFC）、铁离子还原能力（FRAP）、ABTS和DPPH自由基清除活性。然而，乌墨的汤剂在抗AGEs形成（IC50为9.75±0.32mg/mL）、糖化逆转（EC50为66.45±4.51mg/mL）以及抗氧化潜力方面，相对于其他三种植物材料的汤剂具有显著更高（p<0.05）的水平。
乌墨树皮提取物被确定为控制AGEs过度形成的最佳来源。此外，其他三种植物提取物也可有效用作潜在治疗剂，以控制与AGEs介导的健康并发症相关的病理状况。
