Pang Xinyi, Feng Zeyin, Song Xiaoye, Sun Jing, Li Xiangfei, Lu Zhaoxin, Lu Yingjian
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210023, China.
Probiotics Antimicrob Proteins. 2025 Aug 6. doi: 10.1007/s12602-025-10640-0.
Staphylococcus aureus is a common foodborne pathogen, and its prevention and control has always been a hot topic of concern. The purpose of this study was to screen bacteriocin-producing lactic acid bacteria (LAB) and explore its antibacterial and antibiofilm activity against S. aureus. A total of 67 strains of lactic acid bacteria were isolated from fermented foods, and 9 strains with good antibacterial activity against S. aureus and Escherichia coli were screened out according to the inhibition zone experiment and identified by 16S rDNA sequencing. After acid neutralization, and catalase and protease treatment, it was found that Lactiplantibacillus plantarum 65 could produce a new bacteriocin. The cell-free supernatant (CFS) of L. plantarum 65 was separated and purified by organic solvent extraction, sephadex LH20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The antibacterial active peptide obtained was a new type of bacteriocin with an amino acid sequence of KVKPLHA and a molecular weight of 791.5Da. The CFS of L. plantarum 65 showed antibiofilm activity against S. aureus and Listeria monocytogenes, with the highest inhibition rate reaching 83.07%. L. plantarum 65 isolated from fermented food products could produce novel bacteriocin with the ability to inhibit growth and biofilm formation of S. aureus. IMPACT STATEMENT: The obtained results highlight the potential of bacteriocin-producing L. plantarum 65 have dual potential for antibacterial and antibiofilm activity against S. aureus, and it can be used for the prevention and control contamination of S. aureus in food.
金黄色葡萄球菌是一种常见的食源性病原体，其预防和控制一直是备受关注的热点话题。本研究的目的是筛选产细菌素的乳酸菌（LAB），并探讨其对金黄色葡萄球菌的抗菌和抗生物膜活性。从发酵食品中分离出67株乳酸菌，根据抑菌圈实验筛选出9株对金黄色葡萄球菌和大肠杆菌具有良好抗菌活性的菌株，并通过16S rDNA测序进行鉴定。经过酸中和、过氧化氢酶和蛋白酶处理后，发现植物乳杆菌65能够产生一种新的细菌素。植物乳杆菌65的无细胞上清液（CFS）通过有机溶剂萃取、葡聚糖LH20和反相高效液相色谱（RP-HPLC）进行分离纯化。得到的抗菌活性肽是一种新型细菌素，氨基酸序列为KVKPLHA，分子量为791.5Da。植物乳杆菌65的CFS对金黄色葡萄球菌和单核细胞增生李斯特菌具有抗生物膜活性，最高抑制率达到83.07%。从发酵食品中分离出的植物乳杆菌65能够产生新型细菌素，具有抑制金黄色葡萄球菌生长和生物膜形成的能力。影响声明：所得结果突出了产细菌素的植物乳杆菌65对金黄色葡萄球菌具有抗菌和抗生物膜活性的双重潜力，可用于预防和控制食品中金黄色葡萄球菌的污染。
