Dawoud Turki M, Yassin Mohamed A, El-Samawaty Abdel Rahim M, Elgorban Abdallah M
Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
Saudi J Biol Sci. 2021 Mar;28(3):1847-1852. doi: 10.1016/j.sjbs.2020.12.036. Epub 2020 Dec 29.
In this investigation, an alternate green-route based on myco-synthesised silver nanoparticles (Ag NPs) was evaluated to control plant disease to reduce the usage of synthetic chemicals. Here, we described biologically synthesised Ag NPs using the corn grain contaminant, , and were well-characterised by UV-visible spectrophotometer, X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Energy Dispersive Spectroscopy (EDS) and particle size analyzer. The pathogenic behaviour of the spp. were checked on Giza 86 and Giza 90 cultivars under greenhouse conditions. and exhibited high pathogenecity against Giza 90 and Giza 86 cultivars respectively. The antifungal activity of biosynthesised Ag NPs was evaluated against eight species of causing damping-off of cotton seedlings. treatments with different concentrations of Ag NPs were achieved on Czapek Dox agar and Potato dextrose agar plates. Fungal growth was drastically retarded from 25 to 200 ppm of Ag NPs interaction. The antifungal activity of Ag NPs against the spp. was clearly proven.
在本研究中，对基于真菌合成银纳米颗粒（Ag NPs）的替代绿色途径进行了评估，以控制植物病害，减少合成化学品的使用。在此，我们描述了使用玉米籽粒污染物生物合成的Ag NPs，并通过紫外可见分光光度计、X射线粉末衍射（XRD）、透射电子显微镜（TEM）、能量色散光谱（EDS）和粒度分析仪对其进行了充分表征。在温室条件下，检测了 spp. 对吉萨86和吉萨90品种的致病行为。 和 分别对吉萨90和吉萨86品种表现出高致病性。评估了生物合成的Ag NPs对导致棉花幼苗猝倒的八种 物种的抗真菌活性。在查氏琼脂平板和马铃薯葡萄糖琼脂平板上用不同浓度的Ag NPs进行处理。从25到200 ppm的Ag NPs相互作用下，真菌生长受到显著抑制。Ag NPs对 spp. 的抗真菌活性得到了明确证明。
