Sen Research Group, Biochemistry Department, Faculty of Arts and Science, Dumlupinar University, Evliya Celebi Campus, 43100, Kutahya, Turkey.
Food Processing Programme, Technical Science Vocational School, Mersin University, TR-33343, Yenisehir, Mersin, Turkey.
J Pharm Biomed Anal. 2020 Jan 30;178:112970. doi: 10.1016/j.jpba.2019.112970. Epub 2019 Nov 4.
In recent years, the synthesis of nanoparticles via biological processes has attracted considerable attention. The use of plants and plant extracts is one of the most preferred methods for biological synthesis due to their rich biologically active metabolites. In this study, silver nanoparticles (Ag NPs) were synthesized using reishi mushroom (Ganoderma lucidum) extract. Different analytical techniques including X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis spectroscopy, and Fourier Transform Infrared Spectrophotometer (FTIR) were used for the characterization of Ag NPs. UV-vis spectrum exhibited a broad absorption peak between 400-460 nm which indicates the existence of Ag NPs. TEM images showed Ag NPs are spherical with a diameter range of 15-22 nm. In addition, it is shown that Ag NPs form a face-centered cubic structure according to XRD characterization technique. The antioxidant activity towards to 1-Diphenyl-2-picrylhydrazyl (DPPH) was also studied. The highest DPPH scavenging percentage was recorded as 76.45% at 250 mg/L. The DNA cleavage activity results indicated that the green Ag NPs caused single strain DNA cleavage activity for 30 and 60 min at 50 and 100 mg/L, respectively. The Ag NPs antimicrobial activity was also investigated and results recorded as minimum inhibition concentration (MIC). Ag NPs showed a strong antibacterial effect against gram-positive (S. aureus, E. hirae, B. cereus) and gram-negative (E. coli, P. aeruginosa, L. pneumophila subsp. Pneumophila) bacteria. Furthermore, Ag NPs have also been shown to have a high antifungal effect against C. albicans fungus.
近年来，通过生物过程合成纳米粒子引起了相当大的关注。由于植物具有丰富的生物活性代谢物，因此使用植物和植物提取物是生物合成的最优选方法之一。在这项研究中，使用灵芝（Ganoderma lucidum）提取物合成了银纳米粒子（Ag NPs）。使用包括 X 射线光电子能谱（XPS）、X 射线衍射（XRD）、透射电子显微镜（TEM）、紫外-可见光谱和傅里叶变换红外光谱（FTIR）在内的不同分析技术对 Ag NPs 进行了表征。紫外-可见光谱显示在 400-460nm 之间有一个宽吸收峰，这表明存在 Ag NPs。TEM 图像显示 Ag NPs 为球形，直径范围为 15-22nm。此外，根据 XRD 特征技术，表明 Ag NPs 形成面心立方结构。还研究了 Ag NPs 对 1-二苯基-2-苦基肼基（DPPH）的抗氧化活性。在 250mg/L 时，记录到最高的 DPPH 清除率为 76.45%。DNA 切割活性结果表明，在 50 和 100mg/L 时，绿色 Ag NPs 分别在 30 和 60min 内引起单链 DNA 切割活性。还研究了 Ag NPs 的抗菌活性，并记录为最小抑制浓度（MIC）。Ag NPs 对革兰氏阳性（金黄色葡萄球菌、藤黄微球菌、蜡状芽孢杆菌）和革兰氏阴性（大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌亚种。铜绿假单胞菌）细菌表现出很强的抗菌作用。此外，Ag NPs 还表现出对白色念珠菌真菌的高抗真菌作用。
