干燥的L.根茎乙醇提取物的神经保护作用
Neuroprotective effects of ethanolic extract from dry L. rhizomes.
作者信息
Kim Kwan Joong, Jung Young Sung, You Dong Min, Lee Seung Hyun, Lee Guemsan, Kwon Kang-Beom, Kim Dae-Ok
机构信息
Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104 Republic of Korea.
Korea Food Research Institute, Wanju, 55365 Republic of Korea.
出版信息
Food Sci Biotechnol. 2021 Feb 5;30(2):287-297. doi: 10.1007/s10068-020-00868-7. eCollection 2021 Feb.
UNLABELLED
L. rhizome has been used as a traditional medicine to treat fatigue, depression, and cognitive dysfunction. We aimed to authenticate L. rhizome using the DNA barcoding technique and to quantify its main compounds, total phenolics, total flavonoids, and antioxidant capacity, and then to investigate their neuroprotective effects. The sequences of internal transcribed spacer and of L. rhizomes showed a 99% identity with those of NCBI GenBank database according to BLAST searches. Analysis using reversed-phase HPLC revealed five main compounds in L. rhizome. L. rhizome and two bioactive compounds, salidroside and tyrosol, showed free radical scavenging activity. L rhizome and its identified compounds protected neuronal PC-12 cells against oxidative stress and showed moderate acetylcholinesterase inhibition. Taken together, these results suggest that L rhizomes with bioactives can be used as a functional ingredient with potential for neuroprotection.
SUPPLEMENTARY INFORMATION
The online version of this article (doi:10.1007/s10068-020-00868-7) contains supplementary material, which is available to authorized users.
未标注
L. 根茎一直被用作传统药物来治疗疲劳、抑郁和认知功能障碍。我们旨在使用DNA条形码技术对L. 根茎进行鉴定，并对其主要化合物、总酚类、总黄酮类和抗氧化能力进行定量，然后研究它们的神经保护作用。根据BLAST搜索，L. 根茎的内转录间隔区序列与NCBI基因库数据库中的序列显示出99%的同一性。使用反相高效液相色谱法分析发现L. 根茎中有五种主要化合物。L. 根茎以及两种生物活性化合物红景天苷和酪醇表现出自由基清除活性。L. 根茎及其鉴定出的化合物可保护神经元PC-12细胞免受氧化应激，并表现出适度的乙酰胆碱酯酶抑制作用。综上所述，这些结果表明含有生物活性成分的L. 根茎可作为具有神经保护潜力的功能性成分。
补充信息
本文的在线版本（doi:10.1007/s10068-020-00868-7）包含补充材料，授权用户可获取。
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