Gong Xuerui, Feng Shilun, Qiao Zhen, Chen Yu-Cheng
School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Ave., 639798, Singapore.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, Shanghai 200050, China.
Anal Chem. 2021 Apr 13;93(14):5823-5830. doi: 10.1021/acs.analchem.1c00020. Epub 2021 Mar 18.
Optofluidic biolasers have emerged as promising tools for biomedical analysis due to their strong light-matter interactions and miniaturized size. Recent developments in optofluidic lasers have opened a new Frontier in monitoring biological processes. However, most biolasers require precise recording of the lasing spectrum at the single cavity level, which limits its application in high-throughput applications. Herein, a microdroplet laser array encapsulated with living was printed on highly reflective mirrors, where laser emission images were employed to reflect the dynamic changes in living organisms. The concept of image-based lasing analysis was proposed by quantifying the integrated pixel intensity of the lasing image from whispering-gallery modes. Finally, dynamic interactions between and antibiotic drugs were compared under fluorescence and laser emission images. The amplification that occurred during laser generation enabled the quantification of tiny biological changes in the gain medium. Laser imaging presented a significant increase in integrated pixel intensity by 2 orders of magnitude. Our findings demonstrate that image-based lasing analysis is more sensitive to dynamic changes than fluorescence analysis, paving the way for high-throughput on-chip laser analysis of living organisms.
由于其强烈的光与物质相互作用和小型化尺寸，光流控生物激光器已成为生物医学分析中有前景的工具。光流控激光器的最新进展为监测生物过程开辟了新的前沿领域。然而，大多数生物激光器需要在单腔水平精确记录激光光谱，这限制了其在高通量应用中的应用。在此，一种封装有活细胞的微滴激光阵列被打印在高反射镜上，利用激光发射图像来反映活生物体中的动态变化。通过量化来自回音壁模式的激光图像的积分像素强度，提出了基于图像的激光分析概念。最后，在荧光和激光发射图像下比较了细胞与抗生素药物之间的动态相互作用。激光产生过程中发生的放大作用使得能够对增益介质中的微小生物变化进行量化。激光成像显示积分像素强度显著增加了2个数量级。我们的研究结果表明，基于图像的激光分析对动态变化比荧光分析更敏感，为活生物体的高通量片上激光分析铺平了道路。
