Ueki Atsuko, Tonouchi Akio, Kaku Nobuo, Ueki Katsuji
Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka, Yamagata 997-8555, Japan.
Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan.
Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004761. Epub 2021 Mar 18.
Biological soil disinfestation (BSD) or reductive soil disinfestation (RSD) is a bioremediation method used to suppress or eliminate soil-borne plant pathogens by stimulating activities of indigenous anaerobic bacteria of the soil. An anaerobic bacterial strain (TW1) was isolated from an anoxic soil sample subjected to the BSD treatment and comprehensively characterized. Cells of the strain were Gram-stain-positive, slightly curved and motile rods producing terminal spores. The strain was aerotolerant. Strain TW1 was saccharolytic and produced acetate, butyrate, H and CO as fermentation end products. Strain TW1 decomposed β-1,3-glucan (curdlan and laminarin) and degraded mycelial cells of an ascomycete plant pathogen. Major cellular fatty acids of strain TW1 were C, C dimethylacetal (DMA), C aldehyde and C DMA. Strain TW1 made a group on the phylogenetic tree constructed based on 16S rRNA gene sequences with species such as (96.3 %) and (96.0 %). Whole genome analysis of strain TW1 showed that the total length of the genome was 5.28 Mb with the DNA G+C content of 31.3 mol%. The average nucleotide identity (ANIb) between strain TW1 and was 71.2 %. Presence of the genes encoding laminarinase or GH16 β-glucosidase was confirmed from the genome analysis of strain TW1. Based on the genomic, phylogenetic and phenotypic properties obtained, we propose strain TW1 should be assigned in the genus in the family as sp. nov. The type strain TW1 (=NBRC 112097=DSM 110791).
生物土壤消毒（BSD）或还原性土壤消毒（RSD）是一种生物修复方法，通过刺激土壤中本土厌氧细菌的活性来抑制或消除土壤传播的植物病原体。从经过BSD处理的缺氧土壤样本中分离出一株厌氧细菌菌株（TW1）并进行了全面表征。该菌株的细胞革兰氏染色呈阳性，为稍弯曲的能动杆菌，产生端生孢子。该菌株具有耐氧性。菌株TW1可进行糖酵解，产生乙酸盐、丁酸盐、氢气和二氧化碳作为发酵终产物。菌株TW1可分解β-1,3-葡聚糖（可德胶和海带多糖）并降解一种子囊菌植物病原体的菌丝细胞。菌株TW1的主要细胞脂肪酸为C、C二甲基缩醛（DMA）、C醛和C DMA。在基于16S rRNA基因序列构建的系统发育树中，菌株TW1与诸如（96.3%）和（96.0%）等物种聚为一类。菌株TW1的全基因组分析表明，基因组总长度为5.28 Mb，DNA G+C含量为31.3 mol%。菌株TW1与之间的平均核苷酸同一性（ANIb）为71.2%。从菌株TW1的基因组分析中证实了编码海带多糖酶或GH16β-葡萄糖苷酶的基因的存在。基于所获得的基因组、系统发育和表型特征，我们提议将菌株TW1归为科属中的新物种。模式菌株TW1（=NBRC 112097=DSM 110791）。
