Flaiz Maximilian, Baur Tina, Brahner Sven, Poehlein Anja, Daniel Rolf, Bengelsdorf Frank R
Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4269-4279. doi: 10.1099/ijsem.0.004283.
A strictly anaerobic bacterial strain designated EA1 was isolated from an enrichment culture inoculated with biogas reactor content. Cells of strain EA1 are spore-forming rods (1-3×0.4-0.8 µm) and stain Gram-negative, albeit they possess a Gram-positive type of cell-wall ultrastructure. Growth of strain EA1 was observed at 30 and 37 °C and within a pH range of pH 5-9. The major components recovered in the fatty acid fraction were C, C, C DMA (dimethyl acetal) and C 7. Strain EA1 fermented several mono- and disaccharides. Metabolic end products from fructose were acetate, butyrate, caproate and lactate. Furthermore, ethanol, CO and H were identified as products. The genome consists of a chromosome (3.9 Mbp) with 3797 predicted protein-encoding genes and a G+C content of 51.25 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EA1 represents a novel taxon within the family . The most closely related type strains of EA1, based on 16S rRNA gene sequence identity, are BS-1 (94.9 %), [] DSM 753 (93.8 %), [] DSM 1294 (91.7 %) and ATCC 27255 (91.0 %). Further phenotypic characteristics of strain EA1 differentiate it from related, validly described bacterial species. Strain EA1 represents a novel genus and novel species within the family . The proposed name is gen. nov., sp. nov. The type strain is EA1 (DSM 107079=JCM 33110).
从接种沼气反应器内容物的富集培养物中分离出一株严格厌氧的细菌菌株，命名为EA1。EA1菌株的细胞为产芽孢杆菌（1-3×0.4-0.8微米），革兰氏染色阴性，但其细胞壁超微结构为革兰氏阳性类型。在30和37℃以及pH 5-9的范围内观察到EA1菌株的生长。脂肪酸组分中回收的主要成分是C、C、C二甲基缩醛（dimethyl acetal）和C 7。EA1菌株发酵多种单糖和双糖。果糖的代谢终产物是乙酸盐、丁酸盐、己酸盐和乳酸盐。此外，还鉴定出乙醇、CO和H作为产物。基因组由一条染色体（3.9 Mbp）组成，有3797个预测的蛋白质编码基因，G+C含量为51.25摩尔%。基于16S rRNA基因序列的系统发育分析表明，EA1菌株代表该科内的一个新分类单元。基于16S rRNA基因序列同一性，与EA1最密切相关的模式菌株是BS-1（94.9%）、[] DSM 753（93.8%）、[] DSM 1294（91.7%）和ATCC 27255（91.0%）。EA1菌株的进一步表型特征使其与相关的、已有效描述的细菌物种区分开来。EA1菌株代表该科内的一个新属和新物种。提议的名称是gen. nov., sp. nov.。模式菌株是EA1（DSM 107079=JCM 33110）。
