• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

环状 RNA_0000326 的沉默通过增强 microRNA-338-3p 依赖性 CDK4 的下调来抑制宫颈癌细胞的增殖、迁移和侵袭。

Silencing of circular RNA_0000326 inhibits cervical cancer cell proliferation, migration and invasion by boosting microRNA-338-3p-dependent down-regulation of CDK4.

机构信息

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, P. R. China.

Academy of Medical Science, Zhengzhou University, Zhengzhou 450052, P. R. China.

出版信息

Aging (Albany NY). 2021 Mar 17;13(6):9119-9134. doi: 10.18632/aging.103711.

DOI:10.18632/aging.103711
PMID:33735107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8034888/
Abstract

Cervical cancer is one of the leading causes of cancer-related death among women, which is attributed partly by limited treatment options. Recent studies have provided in-depth explanations regarding the role of circular RNA in cancers. We aimed to investigate the role of circular RNA_0000326 in cervical cancer. Bioinformatics analysis revealed a high circ_0000326 expression in cervical cancer. Cervical cancer cells and tissues were also observed to have elevated levels of circ_0000326 and the upregulation of circ_0000326 depended on the stage of cancer. Transfection with siRNA of circ_0000326 resulted in the inhibition of proliferation, migration and cell cycle of cancer cells. Interestingly, we confirmed that circ_0000326 served as a sponge for microRNA-338-3p and that the miRNA bound to Cyclin-dependent kinase 4. In the presence of microRNA-338-3p mimic or silencing of circ_0000326, Cyclin-dependent kinase 4 expression was decreased. Transfection with microRNA-338-3p mimic inhibited cell clone formation and proliferation. Moreover, experiment revealed that the injection of shRNA-circ_0000326 lentivirus suppressed tumor growth and decreased Cyclin-dependent kinase 4 expression. Taken altogether, our results showed that circ_0000326 exerted oncogenic effects on cervical cancer by upregulating Cyclin-dependent kinase 4 sponging microRNA-338-3p. This systematic investigation on circ_0000326 could provide further insight into cervical cancer.

摘要

宫颈癌是导致女性癌症相关死亡的主要原因之一，部分原因是治疗选择有限。最近的研究深入解释了环状 RNA 在癌症中的作用。我们旨在研究环状 RNA_0000326 在宫颈癌中的作用。生物信息学分析显示宫颈癌中 circ_0000326 表达较高。还观察到宫颈癌细胞和组织中 circ_0000326 水平升高，circ_0000326 的上调取决于癌症的阶段。用 siRNA 转染 circ_0000326 导致癌细胞增殖、迁移和细胞周期受到抑制。有趣的是，我们证实 circ_0000326 充当 microRNA-338-3p 的海绵，miRNA 与细胞周期蛋白依赖性激酶 4 结合。在 microRNA-338-3p 模拟物存在或 circ_0000326 沉默的情况下，细胞周期蛋白依赖性激酶 4 的表达减少。转染 microRNA-338-3p 模拟物抑制细胞克隆形成和增殖。此外，实验表明，shRNA-circ_0000326 慢病毒的转染抑制肿瘤生长并降低细胞周期蛋白依赖性激酶 4 的表达。总的来说，我们的结果表明 circ_0000326 通过上调 Cyclin-dependent kinase 4 海绵 microRNA-338-3p 对宫颈癌发挥致癌作用。对 circ_0000326 的系统研究可为宫颈癌提供进一步的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/6e81d0590ff4/aging-13-103711-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/e8faca30b74b/aging-13-103711-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/9684e14ecd86/aging-13-103711-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/f509f7d1be2e/aging-13-103711-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/51737cfdd363/aging-13-103711-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/86c1921c1322/aging-13-103711-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/27425ad3588a/aging-13-103711-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/1ed48202107a/aging-13-103711-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/6e81d0590ff4/aging-13-103711-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/e8faca30b74b/aging-13-103711-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/9684e14ecd86/aging-13-103711-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/f509f7d1be2e/aging-13-103711-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/51737cfdd363/aging-13-103711-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/86c1921c1322/aging-13-103711-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/27425ad3588a/aging-13-103711-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/1ed48202107a/aging-13-103711-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1ed/8034888/6e81d0590ff4/aging-13-103711-g008.jpg

相似文献

1
Silencing of circular RNA_0000326 inhibits cervical cancer cell proliferation, migration and invasion by boosting microRNA-338-3p-dependent down-regulation of CDK4.环状 RNA_0000326 的沉默通过增强 microRNA-338-3p 依赖性 CDK4 的下调来抑制宫颈癌细胞的增殖、迁移和侵袭。
Aging (Albany NY). 2021 Mar 17;13(6):9119-9134. doi: 10.18632/aging.103711.
2
Hsa_circ_0031288/hsa-miR-139-3p/Bcl-6 regulatory feedback circuit influences the invasion and migration of cervical cancer HeLa cells.Hsa_circ_0031288/hsa-miR-139-3p/Bcl-6 调控反馈环路影响宫颈癌 HeLa 细胞的侵袭和迁移。
J Cell Biochem. 2020 Oct;121(10):4251-4260. doi: 10.1002/jcb.29650. Epub 2020 Apr 10.
3
Silencing of hsa_circ_0009035 Suppresses Cervical Cancer Progression and Enhances Radiosensitivity through MicroRNA 889-3p-Dependent Regulation of HOXB7.hsa_circ_0009035 的沉默通过 microRNA 889-3p 依赖调控 HOXB7 抑制宫颈癌进展并增强放射敏感性。
Mol Cell Biol. 2021 May 21;41(6):e0063120. doi: 10.1128/MCB.00631-20.
4
Overexpression of circular RNA hsa_circ_0001038 promotes cervical cancer cell progression by acting as a ceRNA for miR-337-3p to regulate cyclin-M3 and metastasis-associated in colon cancer 1 expression.环状 RNA hsa_circ_0001038 的过表达通过作为 miR-337-3p 的 ceRNA 来调节 cyclin-M3 和结肠癌转移相关蛋白 1 的表达，促进宫颈癌细胞的进展。
Gene. 2020 Apr 5;733:144273. doi: 10.1016/j.gene.2019.144273. Epub 2019 Dec 3.
5
Circular RNA hsa_circ_0000515 acts as a miR-326 sponge to promote cervical cancer progression through up-regulation of ELK1.环状RNA hsa_circ_0000515作为miR-326的海绵，通过上调ELK1促进宫颈癌进展。
Aging (Albany NY). 2019 Nov 26;11(22):9982-9999. doi: 10.18632/aging.102356.
6
Knockdown of circ_0006528 Suppresses Cell Proliferation, Migration, Invasion, and Adriamycin Chemoresistance via Regulating the miR-1236-3p/CHD4 Axis in Breast Cancer.circ_0006528 通过调控 miR-1236-3p/CHD4 轴抑制乳腺癌细胞增殖、迁移、侵袭和阿霉素化疗耐药性。
J Surg Res. 2021 Apr;260:104-115. doi: 10.1016/j.jss.2020.10.031. Epub 2020 Dec 14.
7
Circular RNA has_circ_0000034 accelerates retinoblastoma advancement through the miR-361-3p/ADAM19 axis.环状 RNA 通过 miR-361-3p/ADAM19 轴促进视网膜母细胞瘤进展。
Mol Cell Biochem. 2021 Jan;476(1):69-80. doi: 10.1007/s11010-020-03886-5. Epub 2020 Aug 25.
8
Circular RNA circ_HN1 facilitates gastric cancer progression through modulation of the miR-302b-3p/ROCK2 axis.环状 RNA circ_HN1 通过调节 miR-302b-3p/ROCK2 轴促进胃癌进展。
Mol Cell Biochem. 2021 Jan;476(1):199-212. doi: 10.1007/s11010-020-03897-2. Epub 2020 Sep 19.
9
Downregulation of hsa_circ_0007534 restricts the proliferation and invasion of cervical cancer through regulating miR-498/BMI-1 signaling.hsa_circ_0007534 的下调通过调节 miR-498/BMI-1 信号通路限制宫颈癌的增殖和侵袭。
Life Sci. 2019 Oct 15;235:116785. doi: 10.1016/j.lfs.2019.116785. Epub 2019 Aug 21.
10
Circular RNA ZNF609 functions as a competing endogenous RNA in regulating E2F transcription factor 6 through competitively binding to microRNA-197-3p to promote the progression of cervical cancer progression.环状 RNA ZNF609 通过竞争性结合 microRNA-197-3p 作为竞争内源性 RNA 调节 E2F 转录因子 6，从而促进宫颈癌的进展。
Bioengineered. 2021 Dec;12(1):927-936. doi: 10.1080/21655979.2021.1896116.

引用本文的文献

1
Circular RNAs and Cancers.环状RNA与癌症
Adv Exp Med Biol. 2025;1485:273-327. doi: 10.1007/978-981-96-9428-0_18.
2
Circular RNAs in Cell Cycle Regulation of Cancers.环状 RNA 在癌症细胞周期调控中的作用。
Int J Mol Sci. 2024 May 31;25(11):6094. doi: 10.3390/ijms25116094.
3
CircTMEM165 facilitates endothelial repair by modulating mitochondrial fission via miR-192/SCP2 and .环状跨膜蛋白165通过微小RNA-192/固醇载体蛋白2调节线粒体分裂促进内皮修复。

本文引用的文献

1
Recent Progress on Circular RNA Research in Acute Myeloid Leukemia.急性髓系白血病环状RNA研究的最新进展
Front Oncol. 2019 Nov 6;9:1108. doi: 10.3389/fonc.2019.01108. eCollection 2019.
2
MicroRNA-338-3p suppresses cell proliferation, migration and invasion in human malignant melanoma by targeting MACC1.微小RNA-338-3p通过靶向MACC1抑制人恶性黑色素瘤细胞的增殖、迁移和侵袭。
Exp Ther Med. 2019 Aug;18(2):997-1004. doi: 10.3892/etm.2019.7644. Epub 2019 Jun 4.
3
Transforming activity of an oncoprotein-encoding circular RNA from human papillomavirus.
iScience. 2024 Mar 16;27(4):109502. doi: 10.1016/j.isci.2024.109502. eCollection 2024 Apr 19.
4
A review on the role of cyclin dependent kinases in cancers.细胞周期蛋白依赖性激酶在癌症中的作用综述。
Cancer Cell Int. 2022 Oct 20;22(1):325. doi: 10.1186/s12935-022-02747-z.
5
A peptide translated from circPPP1R12A promotes the malignancy of non-small cell lung cancer cells through AKT signaling pathway.环状 RNA PPP1R12A 翻译出的肽通过 AKT 信号通路促进非小细胞肺癌细胞的恶性转化。
J Clin Lab Anal. 2022 Oct;36(10):e24644. doi: 10.1002/jcla.24644. Epub 2022 Aug 21.
6
Oxymatrine induces anti-tumor response in cervical cancer by modulating circ_0008460/miR-197-3p/ribonucleotide reductase subunit M2 (RRM2).氧化苦参碱通过调节 circ_0008460/miR-197-3p/核糖核苷酸还原酶亚基 M2（RRM2）诱导宫颈癌的抗肿瘤反应。
Bioengineered. 2022 May;13(5):12912-12926. doi: 10.1080/21655979.2022.2078943.
7
A Novel Circular RNA CircRFX3 Serves as a Sponge for MicroRNA-587 in Promoting Glioblastoma Progression via Regulating PDIA3.一种新型环状RNA CircRFX3通过调控PDIA3作为微小RNA-587的海绵，促进胶质母细胞瘤进展。
Front Cell Dev Biol. 2021 Dec 7;9:757260. doi: 10.3389/fcell.2021.757260. eCollection 2021.
8
MicroRNA-98-5p modulates cervical cancer progression via controlling PI3K/AKT pathway.微小 RNA-98-5p 通过调控 PI3K/AKT 通路调节宫颈癌进展。
Bioengineered. 2021 Dec;12(2):10596-10607. doi: 10.1080/21655979.2021.2000722.
9
Circular RNA_0000326 promotes bladder cancer progression via microRNA-338-3p/ETS Proto-Oncogene 1/phosphoinositide-3 kinase/Akt pathway.环状 RNA_0000326 通过 microRNA-338-3p/ETS 原癌基因 1/磷酸肌醇 3-激酶/蛋白激酶 B 通路促进膀胱癌进展。
Bioengineered. 2021 Dec;12(2):11410-11422. doi: 10.1080/21655979.2021.2008738.
10
Construction of the circRNA-miRNA-mRNA Regulatory Network of an Abdominal Aortic Aneurysm to Explore Its Potential Pathogenesis.构建腹主动脉瘤的 circRNA-miRNA-mRNA 调控网络，探索其潜在的发病机制。
Dis Markers. 2021 Nov 5;2021:9916881. doi: 10.1155/2021/9916881. eCollection 2021.
一种人乳头瘤病毒编码的环状 RNA 的致癌蛋白转化活性。
Nat Commun. 2019 May 24;10(1):2300. doi: 10.1038/s41467-019-10246-5.
4
Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.瑞博西利，一种选择性细胞周期蛋白依赖性激酶 4/6 抑制剂，能够抑制人宫颈癌的体外和体内增殖并诱导其凋亡。
Biomed Pharmacother. 2019 Apr;112:108602. doi: 10.1016/j.biopha.2019.108602. Epub 2019 Feb 18.
5
Cervical cancer.宫颈癌。
Lancet. 2019 Jan 12;393(10167):169-182. doi: 10.1016/S0140-6736(18)32470-X.
6
MicroRNA-338-3p inhibits tumor growth and metastasis in osteosarcoma cells by targeting RUNX2/CDK4 and inhibition of MAPK pathway.微小 RNA-338-3p 通过靶向 RUNX2/CDK4 和抑制 MAPK 通路抑制骨肉瘤细胞的肿瘤生长和转移。
J Cell Biochem. 2019 Apr;120(4):6420-6430. doi: 10.1002/jcb.27929. Epub 2018 Nov 28.
7
GRSF1-mediated MIR-G-1 promotes malignant behavior and nuclear autophagy by directly upregulating TMED5 and LMNB1 in cervical cancer cells.GRSF1 介导的 MIR-G-1 通过直接上调宫颈癌细胞中的 TMED5 和 LMNB1 促进恶性行为和核自噬。
Autophagy. 2019 Apr;15(4):668-685. doi: 10.1080/15548627.2018.1539590. Epub 2018 Nov 5.
8
LncRNA XLOC_006390 facilitates cervical cancer tumorigenesis and metastasis as a ceRNA against miR-331-3p and miR-338-3p.LncRNA XLOC_006390 作为 ceRNA 对抗 miR-331-3p 和 miR-338-3p，促进宫颈癌的发生和转移。
J Gynecol Oncol. 2018 Nov;29(6):e95. doi: 10.3802/jgo.2018.29.e95.
9
The Biogenesis, Functions, and Challenges of Circular RNAs.环状 RNA 的生成、功能和挑战。
Mol Cell. 2018 Aug 2;71(3):428-442. doi: 10.1016/j.molcel.2018.06.034. Epub 2018 Jul 26.
10
Circular RNA hsa_circ_0023404 exerts an oncogenic role in cervical cancer through regulating miR-136/TFCP2/YAP pathway.环状 RNA hsa_circ_0023404 通过调控 miR-136/TFCP2/YAP 通路在宫颈癌中发挥致癌作用。
Biochem Biophys Res Commun. 2018 Jun 22;501(2):428-433. doi: 10.1016/j.bbrc.2018.05.006. Epub 2018 May 16.